WB (Western Blot)
(Western blot analysis of lysates from Hela, LNCaP cell line, mouse testis and rat testis tissue lysates(from left to right), using ULK3 Antibody (C-term). AAA28641 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Mouse ULK3 Monoclonal Antibody | anti-ULK3 antibody
ULK3 Antibody (C-term)
Reactivity
Human, mouse, rat
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Mouse Monoclonal Antibody (Mab)
Synonyms
ULK3, Antibody; ULK3 Antibody (C-term); Serine/threonine-protein kinase ULK3; Unc-51-like kinase 3; ULK3; anti-ULK3 antibody
Host
Mouse
Reactivity
Human, mouse, rat
Clonality
Monoclonal
Isotype
IgG1,k
Specificity
Generated from a mouse immunized with a KLH conjugated synthetic peptide between 435-468 amino acids from the C-terminal region of human ULK3.
Purity/Purification
Purified Mouse Monoclonal Antibody (Mab)
Form/Format
Supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Sequence Length
472
Applicable Applications for anti-ULK3 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:50
IHC~~1:1000
IHC~~1:1000
Cellular Location
Cytoplasm. Note=Localizes to pre-autophagosomal structure during cellular senescence
Tissue Location
Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optic nerve. In the central nervous system, lowest levels in the spinal cord
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from Hela, LNCaP cell line, mouse testis and rat testis tissue lysates(from left to right), using ULK3 Antibody (C-term). AAA28641 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from Hela, LNCaP cell line, mouse testis and rat testis tissue lysates(from left to right), using ULK3 Antibody (C-term). AAA28641 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis ofparaffin-embedded Human kidney sectionusing Pink1 (AAA28641). AAA28641 wasdiluted at 1:50 dilution. A undilutedbiotinylated goat polyvalent antibody wasused as the secondary, followed by DABstaining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis ofparaffin-embedded Human kidney sectionusing Pink1 (AAA28641). AAA28641 wasdiluted at 1:50 dilution. A undilutedbiotinylated goat polyvalent antibody wasused as the secondary, followed by DABstaining.)
Related Product Information for anti-ULK3 antibody
Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.
Product Categories/Family for anti-ULK3 antibody
References
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Zody M.C.,et al.Nature 440:671-675(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Daub H.,et al.Mol. Cell 31:438-448(2008).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Zody M.C.,et al.Nature 440:671-675(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Daub H.,et al.Mol. Cell 31:438-448(2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 53444
NCBI Official Full Name
serine/threonine-protein kinase ULK3 isoform a
NCBI Official Synonym Full Names
unc-51 like kinase 3
NCBI Official Symbol
ULK3
NCBI Protein Information
serine/threonine-protein kinase ULK3
UniProt Protein Name
Serine/threonine-protein kinase ULK3
UniProt Gene Name
ULK3
UniProt Entry Name
ULK3_HUMAN
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Product Notes
The ULK3 ulk3 (Catalog #AAA28641) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ULK3 Antibody (C-term) reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's ULK3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:50 IHC~~1:1000. Researchers should empirically determine the suitability of the ULK3 ulk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ULK3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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