IHC (Immunohistochemisry)
(Anti- ULK3 Picoband antibody, AAA46279, IHC(P)IHC(P): Human Lung Cancer Tissue)
ULK3 Polyclonal Antibody | anti-ULK3 antibody
Anti-ULK3 Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Synonyms
ULK3, Antibody; Anti-ULK3 Antibody; Serine/threonine-protein kinase ULK3; DKFZP434C131; FLJ90566; Ulk3; ULK3_HUMAN; unc 51 like kinase 3 (C. elegans); Unc-51-like kinase 3; unc-51 like kinase 3; anti-ULK3 antibody
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
472
Applicable Applications for anti-ULK3 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at C-terminus of human ULK3 (427-454aa HTEVQNLMARAEYLKEQVKMRESRWEAD), different from the related mouse and rat sequences by four amino acids.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemisry)
(Anti- ULK3 Picoband antibody, AAA46279, IHC(P)IHC(P): Human Lung Cancer Tissue)
IHC (Immunohistochemisry)
(Anti- ULK3 Picoband antibody, AAA46279, IHC(P)IHC(P): Human Lung Cancer Tissue)
IHC (Immunohiostchemistry)
(Anti- ULK3 Picoband antibody, AAA46279, IHC(P)IHC(P): Rat Brain Tissue)
IHC (Immunohiostchemistry)
(Anti- ULK3 Picoband antibody, AAA46279, IHC(P)IHC(P): Rat Brain Tissue)
WB (Western Blot)
(Anti- ULK3 Picoband antibody, AAA46279, Western blottingAll lanes: Anti ULK3 (AAA46279) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Testis Tissue Lysate at 50ugLane 3: Mouse Brain Tissue Lysate at 50ugLane 4: Human Placenta Tissue Lysate at 50ugLane 5: 22RV1 Whole Cell Lysate at 40ugLane 6: SMMC Whole Cell Lysate at 40ugPredicted bind size: 53KDObserved bind size: 53KD)
WB (Western Blot)
(Anti- ULK3 Picoband antibody, AAA46279, Western blottingAll lanes: Anti ULK3 (AAA46279) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Testis Tissue Lysate at 50ugLane 3: Mouse Brain Tissue Lysate at 50ugLane 4: Human Placenta Tissue Lysate at 50ugLane 5: 22RV1 Whole Cell Lysate at 40ugLane 6: SMMC Whole Cell Lysate at 40ugPredicted bind size: 53KDObserved bind size: 53KD)
Related Product Information for anti-ULK3 antibody
Description: Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase ULK3(ULK3) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Background: Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. In search of potential homologues to Drosophila and human Fu, people have cloned human serine/threonine kinase ULK3 and assessed its ability to regulate GLI transcription factors, mediators of SHH signaling. And ULK3 enhances endogenous and over-expressed GLI1 and GLI2 transcriptional activity in cultured cells, as assessed by GLI-luciferase reporter assay. Besides that, ULK3 alters subcellular localization of GLI1, as assessed by immunofluorescent staining and immunoblotting assays. It is showed that ULK3 is an autophosphorylated kinase and phosphorylates GLI proteins in vitro. Also, ULK3 catalytical activity is crucial for its function in SHH pathway. It is demonstrated that ULK3 is widely expressed and its expression is higher in a number of tissues where Shh signaling is known to be active. The data suggest that serine/threonine kinase ULK3 is involved in the SHH pathway as a positive regulator of GLI proteins.
Background: Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. In search of potential homologues to Drosophila and human Fu, people have cloned human serine/threonine kinase ULK3 and assessed its ability to regulate GLI transcription factors, mediators of SHH signaling. And ULK3 enhances endogenous and over-expressed GLI1 and GLI2 transcriptional activity in cultured cells, as assessed by GLI-luciferase reporter assay. Besides that, ULK3 alters subcellular localization of GLI1, as assessed by immunofluorescent staining and immunoblotting assays. It is showed that ULK3 is an autophosphorylated kinase and phosphorylates GLI proteins in vitro. Also, ULK3 catalytical activity is crucial for its function in SHH pathway. It is demonstrated that ULK3 is widely expressed and its expression is higher in a number of tissues where Shh signaling is known to be active. The data suggest that serine/threonine kinase ULK3 is involved in the SHH pathway as a positive regulator of GLI proteins.
References
1. Maloverjan A; Piirsoo M; Michelson P; Kogerman P; Osterlund T. "Identification of a novel serine/threonine kinase ULK3 as a positive regulator of Hedgehog pathway". Exp Cell Res, 2010 Feb 15.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,997 Da
NCBI Official Full Name
serine/threonine-protein kinase ULK3 isoform a
NCBI Official Synonym Full Names
unc-51 like kinase 3
NCBI Official Symbol
ULK3
NCBI Protein Information
serine/threonine-protein kinase ULK3
UniProt Protein Name
Serine/threonine-protein kinase ULK3
UniProt Gene Name
ULK3
UniProt Entry Name
ULK3_HUMAN
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Product Notes
The ULK3 ulk3 (Catalog #AAA46279) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-ULK3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ULK3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ULK3 ulk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ULK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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