Mouse anti-Human Uroplakin 1B Monoclonal Antibody | anti-UPK1B antibody

Uroplakin 1B (Urothelial Differentiation Marker)

Cat. Num. AAA215250

• Gene Names: UPK1B; UPIB; UPK1; TSPAN20
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Uroplakin 1B, Antibody; Uroplakin 1B (Urothelial Differentiation Marker); Tetraspanin-20; Tspan-20; TSPAN20; UP1b; UPIb; UPK1B; Uroplakin-1b; anti-UPK1B antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: UPK1B/3273
• Specificity: The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is found in the asymmetrical unit membrane (AUM) where it can form a complex with other transmembrane 4 superfamily proteins. It may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions.UPK1B is expressed by terminally differentiated urothelial cells.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 260

• Applicable Applications for anti-UPK1B antibody: IHC (Immunohistochemistry), ELISA
• Positive Control: Urinary Bladder (IHC).
• Immunogen: Recombinant fragment (around aa 109-229) of human Uroplakin 1B (UPK1B) protein (exact sequence is proprietary)
• Cellular Localization: Membrane.
• Hu-chromosome Location: 3q13.32
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using Monospecific Mouse Monoclonal Antibody to Uroplakin 1B (UPK1B/3273). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

SDS_PAGE

(SDS-PAGE Analysis Purified Uroplakin 1B Mouse Monoclonal Antibody (UPK1B/3273). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemisry)

(Formalin-fixed, paraffin-embedded human Urothelial Carcinoma stained with Monospecific Mouse Monoclonal Antibody to Uroplakin 1B (UPK1B/3273).)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Urothelial Carcinoma stained with Monospecific Mouse Monoclonal Antibody to Uroplakin 1B (UPK1B/3273).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Urothelial Carcinoma stained with Monospecific Mouse Monoclonal Antibody to Uroplakin 1B (UPK1B/3273).)

References

Carpenter AR, et al. Kidney Int. 2016;89(3):612-24.

NCBI and Uniprot Product Information

• NCBI GI #: 49619237
• NCBI GeneID: 7348
• NCBI Accession #: NP_008883
• NCBI GenBank Nucleotide #: NP_008883.2
• UniProt Accession #: O75841
• Molecular Weight: 30kDa
• NCBI Official Full Name: uroplakin-1b
• NCBI Official Synonym Full Names: uroplakin 1B
• NCBI Official Symbol: UPK1B
• NCBI Official Synonym Symbols: UPIB; UPK1; TSPAN20
• NCBI Protein Information: uroplakin-1b
• UniProt Protein Name: Uroplakin-1b
• UniProt Gene Name: UPK1B
• UniProt Synonym Gene Names: TSPAN20; UP1b; Tspan-20; UPIb
• UniProt Entry Name: UPK1B_HUMAN