FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells using Anti-USP11 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.)
Mouse anti-Human USP11 Monoclonal Antibody | anti-USP11 antibody
Anti-USP11 Antibody
Gene Names
USP11; UHX1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
This antibody is purified through a protein G column.
Synonyms
USP11, Antibody; Anti-USP11 Antibody; anti-USP11 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Specificity
Recognizes endogenous levels of USP11 protein.
Purity/Purification
This antibody is purified through a protein G column.
Form/Format
Mouse IgG1 kappa. Liquid in PBS, pH7.3, 30% glycerol, and 0.01% sodium azide.
Applicable Applications for anti-USP11 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human USP11. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells using Anti-USP11 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells using Anti-USP11 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.)
WB (Western Blot)
(Western blot analysis of USP11 expression in Jurkat (A), Hela (B), LNCaP (C) whole cell lysates. (Predicted band size: 109 kD; Observed band size: 110 kD))
WB (Western Blot)
(Western blot analysis of USP11 expression in Jurkat (A), Hela (B), LNCaP (C) whole cell lysates. (Predicted band size: 109 kD; Observed band size: 110 kD))
Related Product Information for anti-USP11 antibody
Mouse monoclonal antibody to USP11
Product Categories/Family for anti-USP11 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
109,817 Da
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 11
NCBI Official Synonym Full Names
ubiquitin specific peptidase 11
NCBI Official Symbol
USP11
NCBI Official Synonym Symbols
UHX1
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 11; ubiquitin thioesterase 11; deubiquitinating enzyme 11; ubiquitin thiolesterase 11; ubiquitin-specific processing protease 11; ubiquitin-specific-processing protease 11; ubiquitin carboxyl-terminal hydrolase, X-lin
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 11
UniProt Gene Name
USP11
UniProt Synonym Gene Names
UHX1
UniProt Entry Name
UBP11_HUMAN
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Product Notes
The USP11 usp11 (Catalog #AAA266044) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-USP11 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's USP11 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the USP11 usp11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP11, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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