WB (Western Blot)
(Detection of human USP11 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, Hep-G2, U2OS, HEK293T, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP11 antibody AAA211553 (lot AAA211553-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human USP11 Polyclonal Antibody | anti-USP11 antibody
Rabbit anti-USP11 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
USP11, Antibody; Rabbit anti-USP11 Antibody, Affinity Purified; ubiquitin thiolesterase 11; UHX1; ubiquitin-specific processing protease 11; ubiquitin thioesterase 11; ubiquitin carboxyl-terminal hydrolase, X-linked; ubiquitin carboxyl-terminal hydrolase 11; deubiquitinating enzyme 11; Ubiquitin-specific-processing protease 11; Ubiquitin carboxyl-terminal hydrolase 11; ubiquitin specific peptidase 11; anti-USP11 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-USP11 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 913 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human USP11 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, Hep-G2, U2OS, HEK293T, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP11 antibody AAA211553 (lot AAA211553-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human USP11 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, Hep-G2, U2OS, HEK293T, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP11 antibody AAA211553 (lot AAA211553-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human USP11 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP11 antibody AAA211553 (lot AAA211553-2) used for IP at 6 ug per reaction. USP11 was also immunoprecipitated by a previous lot of this antibody (lot AAA211553-1). For blotting immunoprecipitated USP11, AAA211553 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human USP11 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP11 antibody AAA211553 (lot AAA211553-2) used for IP at 6 ug per reaction. USP11 was also immunoprecipitated by a previous lot of this antibody (lot AAA211553-1). For blotting immunoprecipitated USP11, AAA211553 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
Related Product Information for anti-USP11 antibody
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 11 (USP11) is a member of this family. USP11 has been found to associate with several proteins and may have functions independent of its deubiquitinating activity. USP11 deubiquitinates and associates with the RanGTP-binding protein, RanBPM. It has also been found to associate with BRCA2 and participate in the BRCA2 DNA damage related pathway; however, it does not appear to be involved in BRCA2 deubiquitination. USP11 also appears to play a role in the regulation of the IKKalpha and p53 signaling pathway by a mechanism independent of it deubiquitination activity.
Product Categories/Family for anti-USP11 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The USP11 usp11 (Catalog #AAA211553) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-USP11 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's USP11 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the USP11 usp11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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