IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells with AAA279033 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
Mouse USP6 Monoclonal Antibody | anti-USP6 antibody
Human anti-Homo sapiens (Human) USP6 Monoclonal antibody
Gene Names
USP6; HRP1; TRE2; TRE17; Tre-2; TRESMCR; USP6-short
Reactivity
African swine fever virus
Applications
Immunofluorescence, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
USP6, Antibody; Human anti-Homo sapiens (Human) USP6 Monoclonal antibody; USP6 Monoclonal Antibody; Deubiquitinating enzyme 6; HRP1; Proto-oncogene TRE-2; TRE17; TRE2; Ubiquitin carboxyl-terminal hydrolase 6; Ubiquitin specific protease 6; Ubiquitin thiolesterase 6; Ubiquitin-specific-processing protease 6; UBP6_HUMAN; USP6; anti-USP6 antibody
Host
Mouse
Reactivity
African swine fever virus
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3C3G2
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid; 0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4.
Applicable Applications for anti-USP6 antibody
IF (Immunofluorescence), WB (Western Blot), ELISA
Immunogen Species
Human
Immunogen
Recombinant Human Ubiquitin carboxyl-terminal hydrolase 6 protein (348-535AA)
Conjugation
Non-conjugated
Research Area
Cell Biology
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells with AAA279033 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells with AAA279033 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
WB (Western Blot)
(Western BlotPositive WB detected in: USP6 antibody at 1:1000Lane 1: MCF-7 whole cell lysateLane 2: THP-1 whole cell lysateLane 3: SH-SY5Y whole cell lysateLane 4: Jurkat whole cell lysateLane 5: HEK293 whole cell lysateLane 6: Hela whole cell lysateSecondaryGoat polyclonal to Mouse IgG at 1/20000 dilutionPredicted band size: 89, 122, 159 KDaObserved band size: 89, 122, 159 KDaExposure time: 1min)
WB (Western Blot)
(Western BlotPositive WB detected in: USP6 antibody at 1:1000Lane 1: MCF-7 whole cell lysateLane 2: THP-1 whole cell lysateLane 3: SH-SY5Y whole cell lysateLane 4: Jurkat whole cell lysateLane 5: HEK293 whole cell lysateLane 6: Hela whole cell lysateSecondaryGoat polyclonal to Mouse IgG at 1/20000 dilutionPredicted band size: 89, 122, 159 KDaObserved band size: 89, 122, 159 KDaExposure time: 1min)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
89,577 Da
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 6
NCBI Official Synonym Full Names
ubiquitin specific peptidase 6
NCBI Official Symbol
USP6
NCBI Official Synonym Symbols
HRP1; TRE2; TRE17; Tre-2; TRESMCR; USP6-short
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 6
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 6
UniProt Gene Name
USP6
UniProt Synonym Gene Names
HRP1; TRE2
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Product Notes
The USP6 usp6 (Catalog #AAA279033) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Human anti-Homo sapiens (Human) USP6 Monoclonal antibody reacts with African swine fever virus and may cross-react with other species as described in the data sheet. AAA Biotech's USP6 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the USP6 usp6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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