Rabbit anti-Human USP6 Polyclonal Antibody | anti-USP6 antibody

USP6 Antibody (C-term)

Cat. Num. AAA288561

• Gene Names: USP6; HRP1; TRE2; TRE17; Tre-2; TRESMCR; USP6-short
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: USP6, Antibody; USP6 Antibody (C-term); Ubiquitin carboxyl-terminal hydrolase 6; Deubiquitinating enzyme 6; Proto-oncogene TRE-2; Ubiquitin thioesterase 6; Ubiquitin-specific-processing protease 6; USP6; HRP1; TRE2; anti-USP6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This USP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1280-1310 amino acids from the C-terminal region of human USP6.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.44 (varies by lot)
• Sequence Positions: 1280-1310
• Sequence Length: 1406

• Applicable Applications for anti-USP6 antibody: FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(USP6 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot)

(USP6 Antibody (E978) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the USP6 antibody detected the USP6 protein (arrow).)

Related Product Information for anti-USP6 antibody

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Product Categories/Family for anti-USP6 antibody

Cell Biology

References

Paulding, C.A., et al., Proc. Natl. Acad. Sci. U.S.A. 100(5):2507-2511 (2003). Papa, F.R., et al., Nature 366(6453):313-319 (1993). Nakamura, T., et al., Oncogene 7(4):733-741 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 109638751
• NCBI GeneID: 9098
• NCBI Accession #: NP_004496.2
• NCBI GenBank Nucleotide #: NM_004505.3
• UniProt Accession #: P35125; Q15634; Q86WP6; Q8IWT4
• Molecular Weight: 158658
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 6
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 6
• NCBI Official Symbol: USP6
• NCBI Official Synonym Symbols: HRP1; TRE2; TRE17; Tre-2; TRESMCR; USP6-short
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 6
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 6
• UniProt Gene Name: USP6
• UniProt Synonym Gene Names: HRP1; TRE2
• UniProt Entry Name: UBP6_HUMAN