WB (Western Blot)
(Western Blot analysis of Rat brain membrane lysate showing detection of VAChT protein using Mouse Anti-VAChT Monoclonal Antibody, Clone S6-38. Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody at 1:1000.)
Mouse VAChT Monoclonal Antibody | anti-SLC18A3 antibody
Anti-VAChT antibody
Gene Names
SLC18A3; VACHT
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, Immunohistochemistry
Purity
Protein G purified
Synonyms
VAChT, Antibody; Anti-VAChT antibody; Vesicular Acetylcholine Transporter; MGC12716; rVAT; Slc18a3; Solute carrier family 18 (vesicular acetylcholine) member 3; Solute carrier family 18 (vesicular monoamine) member 3; Solute carrier family 18 member 3; anti-SLC18A3 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
S6-38
Specificity
Detects ~56kDa
Purity/Purification
Protein G purified
Concentration
1mg/mL (varies by lot)
Sequence Length
532
Applicable Applications for anti-SLC18A3 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), IHC (Immunohistochemistry)
Immunogen
Synthetic peptide amino acids 521-532 of human VAChT
Certificate of Analysis
A dilution of 1:50-1:200 was sufficient for detection of VAChT Transporter in rat brain using immunohistochemistry analysis and goat anti-mouse IgG:HRP as the secondary antibody.
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide.
Cellular Localization
Membrane
Research Area(s)
Neuroscience; Cell Structure; Pre-Synaptic Markers; Pumps/Transporters; Neurotransmitter Transporters
Preparation and Storage
Store at -20°C; 1 year (Long Term); Avoid freeze/thaw cycles
WB (Western Blot)
(Western Blot analysis of Rat brain membrane lysate showing detection of VAChT protein using Mouse Anti-VAChT Monoclonal Antibody, Clone S6-38. Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody at 1:1000.)
WB (Western Blot)
(Western Blot analysis of Rat brain membrane lysate showing detection of VAChT protein using Mouse Anti-VAChT Monoclonal Antibody, Clone S6-38. Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody at 1:1000.)
Related Product Information for anti-SLC18A3 antibody
VAChT is a member of the vesicular amine transporter (VMAT) family. The encoded transmembrane protein transports acetylcholine into secretory vesicle for release into the extracellular space. Acetylcholine (Ach) transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.
Product Categories/Family for anti-SLC18A3 antibody
References
1. Erickson J.D., Varoqui H. (2000) FASEB J. 14(15): 2450-2458. 2. Weihe E., Tao-Cheng J.H., Schafer M.K., Erickson J.D., Eiden L.E. (1996) Proc Natl Acad Sci USA. 93(8): 3547-3552.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~56kDa
NCBI Official Full Name
vesicular acetylcholine transporter
NCBI Official Synonym Full Names
solute carrier family 18 (vesicular acetylcholine transporter), member 3
NCBI Official Symbol
SLC18A3
NCBI Official Synonym Symbols
VACHT
NCBI Protein Information
vesicular acetylcholine transporter; solute carrier family 18 member 3; solute carrier family 18, member 3; solute carrier family 18 (vesicular acetylcholine), member 3
UniProt Protein Name
Vesicular acetylcholine transporter
UniProt Gene Name
SLC18A3
UniProt Synonym Gene Names
VACHT; VAChT
UniProt Entry Name
VACHT_HUMAN
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Product Notes
The SLC18A3 slc18a3 (Catalog #AAA103160) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-VAChT antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's VAChT can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the SLC18A3 slc18a3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VAChT, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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