Mouse anti-Human VEGF-C Monoclonal Antibody | anti-VEGF-C antibody

Mouse Anti-Human VEGF-C

Cat. Num. AAA79091

• Gene Names: VEGFC; VRP; Flt4-L
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein G purified
• Synonyms: VEGF-C, Antibody; Mouse Anti-Human VEGF-C; Anti-human VEGF-C (#9E7); VRP; FLT4-L; VEGF-related protein; anti-VEGF-C antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: IgG_2a; #9E7
• Purity/Purification: Protein G purified
• Form/Format: Lyophilized
• Sequence Length: 419

• Applicable Applications for anti-VEGF-C antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: recombinant human VEGF-C (RT# 300-079)
• Preparation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with recombinant human VEGF-C.
• Buffer: PBS
• Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml
• Preparation and Storage: The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 degree C. Frozen aliquots are stable for at least 6 months when stored at -20 degree C.

IHC (Immunohistochemisry)

(IHC with paraffin-embedded sections of human colon carcinoma tissue. Wroclaw Medical University Department of Histology and Embryology.)

Application Data

Application Data

Related Product Information for anti-VEGF-C antibody

VEGF-C, also known as Vascular Endothelial Growth Factor Related Protein (VRP), is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. The human VEGF-C cDNA encodes a pre-pro-protein of 416 amino acids residues. It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. Recombinant human VEGF-C, lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain, forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT-4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells, it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant human VEGF-C is also a mitogen for vascular endothelial cells, it is much less potent than VEGF-A. The recombinant human VEGF-C contains 115 amino acids residues and was fused to a His-tag (6x His) at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 18-24 kDa protein in SDS-PAGE under reducing conditions.

References

1. Joukov et al., EMBO J 15:290, 1996 2. Olofsson et al., Curr Opin Biotech 10:528, 1999 3. Kukk et al., Development 122:3829, 1996

NCBI and Uniprot Product Information

• NCBI GI #: 4885653
• NCBI GeneID: 7424
• NCBI Accession #: NP_005420.1
• NCBI GenBank Nucleotide #: NM_005429.2
• UniProt Accession #: P49767; B2R9Q8
• NCBI Official Full Name: vascular endothelial growth factor C preproprotein
• NCBI Official Synonym Full Names: vascular endothelial growth factor C
• NCBI Official Symbol: VEGFC
• NCBI Official Synonym Symbols: VRP; Flt4-L
• NCBI Protein Information: vascular endothelial growth factor C; VEGF-C; FLT4 ligand DHM; vascular endothelial growth factor-related protein
• UniProt Protein Name: Vascular endothelial growth factor C
• UniProt Gene Name: VEGFC
• UniProt Synonym Gene Names: VEGF-C; Flt4-L; VRP
• UniProt Entry Name: VEGFC_HUMAN