Rat anti-Mouse VEGFR-2/Flk-1 Monoclonal Antibody | anti-VEGFR-2/Flk-1 antibody

Rat Anti-Mouse VEGFR-2/Flk-1

Cat. Num. AAA79111

• Gene Names: Kdr; Flk1; Ly73; Flk-1; Krd-1; VEGFR2; VEGFR-2; sVEGFR-2; 6130401C07
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Protein G purified
• Synonyms: VEGFR-2/Flk-1, Antibody; Rat Anti-Mouse VEGFR-2/Flk-1; Anti-mouse VEGFR-2/Flk-1-Biotin (#3C8); Vascular endothelial growth factor receptor 2; Protein-tyrosine kinase receptor flk-1; anti-VEGFR-2/Flk-1 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: IgG; 3C8
• Specificity: The unconjugated antibody will detect native mouse VEGFR-2/Flk-1 in Western analysis.
• Purity/Purification: Protein G purified
• Form/Format: Lyophilized
• Sequence Length: 1367

• Applicable Applications for anti-VEGFR-2/Flk-1 antibody: WB (Western Blot)
• Immunogen: murine stromal cell line
• Conjugation: Biotin
• Preparation: Monoclonal antibodies were produced with the help of BALB/c mice using recombinant mouse soluble extracellular Flk-1 as the immunizing antigen
• Buffer/Stabilizer: PBS; 50X BSA
• Preservative: 0.02% sodium azide
• Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
• Warnings: Reagents cont ain sodium azide. Under acidic conditions sodiulll azide yields hydrazoic acid, this is extremely toxic. Azide compounds should be diluted with funning waler before discarding. These precautions are recommended to avoid deposits in plumbing where explosive condition may develop.
• Preparation and Storage: The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 degree C. Frozen aliquots are stable for at least 6 months when stored at -20 degree C.

Application Data

Application Data

Related Product Information for anti-VEGFR-2/Flk-1 antibody

Disruption of the precise balance of positi ve and negative molecular regulators of blood and lymphatic vesse l growth can lead to myriad diseases. Although dozens of natural inhibitors of hemangiogenesis have been identified, an endogenous selective inhibitor of lymphatic vesse l growth has not to our knowledge been previou sly described. A splice variant of the gene encoding vascular endothelial growth factor receptor-2 (VEGFR-2) that encodes a secreted form of the protein. designated endogenous soluble VEGFR-2 (esV EG FR- 2/KDR) has been described. The endogenous soluble esKDR inhibits developmental and reparative Iymphangiogenes is by blocking VEGF-C fun ction. Tissue-specific loss ofesKDR in mice induced, at birth, spontaneous lymphatic invasion of the normally alymphatic cornea and hyperplasia of skin lymphatics without affecting blood vasculature. Administration of esKDR inhibited Iymphangiogenesis but not hemangiogenesis induced by corneal suture injlllY or tran splantation, enhanced corneal allograft survival and suppressed lymphangioma cellular proliferation. Naturally occurring esKDR thus acts as a molecular uncoupler of blood and lymphatic vessels; modulation of esKDR might have therapeutic effects in treating lymphatic vascular malformations, transplantation rejection and potentially, tumor lymphangiogenesis and lymphedema.

References

1. Shibata et al, BMC Medicine 8 (2010) 2. Albaquerque et al, Nature Med 2009 3. Ebos et al, Mol Cancer Res 2 (2004) 4. Ebos et al, Cancer res 68 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 549321
• NCBI GeneID: 16542
• UniProt Accession #: P35918; C5H7S5
• Molecular Weight: 152,517 Da
• NCBI Official Full Name: Vascular endothelial growth factor receptor 2
• NCBI Official Synonym Full Names: kinase insert domain protein receptor
• NCBI Official Symbol: Kdr
• NCBI Official Synonym Symbols: Flk1; Ly73; Flk-1; Krd-1; VEGFR2; VEGFR-2; sVEGFR-2; 6130401C07
• NCBI Protein Information: vascular endothelial growth factor receptor 2; kinase NYK; VEGF receptor-2; fetal liver kinase 1; protein-tyrosine kinase receptor flk-1; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; vascular endothelial growth factor receptor- 2; soluble vascular endothelial growth factor receptor 2
• UniProt Protein Name: Vascular endothelial growth factor receptor 2
• UniProt Gene Name: Kdr
• UniProt Synonym Gene Names: Flk-1; Flk1; VEGFR-2; FLK-1
• UniProt Entry Name: VGFR2_MOUSE