Mouse Vimentin Monoclonal Antibody | anti-VIM antibody
Mouse anti Vimentin, conjugated with FITC
Gene Names
VIM; CTRCT30
Reactivity
Canine, Caprine, Chicken, Hamster, Human, Monkey, Mouse, Rat, Swine, Xenopus, Zebrafish
Applications
Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Synonyms
Vimentin, Antibody; Mouse anti Vimentin, conjugated with FITC; anti-VIM antibody
Host
Mouse
Reactivity
Canine, Caprine, Chicken, Hamster, Human, Monkey, Mouse, Rat, Swine, Xenopus, Zebrafish
Clonality
Monoclonal
Isotype
IgG1
Clone Number
RV202
Specificity
RV202-FITC reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
Form/Format
Each vial contains 1ml FITC-conjugated vimentin monoclonal antibody in PBS containing 0,1% BSA, 0.09% sodium azide. Approximately 100 tests.
Applicable Applications for anti-VIM antibody
IHC (Immunohistochemistry), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry)
Host Note
RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.
Preparation and Storage
The antibody is shipped on blue ice and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Related Product Information for anti-VIM antibody
Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.
Product Categories/Family for anti-VIM antibody
References
Ramaekers, F., Huysmans, A., Schaart, G., Moesker, O., and Vooijs, P. (1987). Tissue distribution of keRatin 7 as monitored by a monoclonal antibody, Exp Cell Res 170, 235-49. Viebahn, C., Lane, E. B., and Ramaekers, F. C. (1988). KeRatin and vimentin expression in early organogenesis of the Rabbit embryo, Cell Tissue Res 253, 553-62. Pieper, F. R., Schaart, G., Krimpenfort, P. J., Henderik, J. B., Moshage, H. J., van de Kemp, A., Ramaekers, F. C., Berns, A., and Bloemendal, H. (1989). Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells, J Cell Biol 108, 1009-24. Raats, J. M., Pieper, F. R., Vree Egberts, W. T., Verrijp, K. N., Ramaekers, F. C., and Bloemendal, H. (1990). Assembly of amino-terminally deleted desmin in vimentin-free cells, J Cell Biol 111, 1971-85. Ramaekers, F., van Niekerk, C., Poels, L., Schaafsma, E., Huijsmans, A., Robben, H., Schaart, G., and Vooijs, P. (1990). Use of monoclonal antibodies to keRatin 7 in the differential diagnosis of adenocarcinomas, Am J Pathol 136, 641-55.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
vimentin
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
VIM
NCBI Official Synonym Symbols
CTRCT30
NCBI Protein Information
vimentin
UniProt Protein Name
Vimentin
UniProt Gene Name
VIM
UniProt Entry Name
VIME_HUMAN
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Product Notes
The VIM vim (Catalog #AAA77503) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Vimentin, conjugated with FITC reacts with Canine, Caprine, Chicken, Hamster, Human, Monkey, Mouse, Rat, Swine, Xenopus, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's Vimentin can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the VIM vim for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vimentin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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