Mouse anti-Human VTCN1 / B7-H4 Monoclonal Antibody | anti-VTCN1 antibody

Mouse Monoclonal [clone H74] (IgG1,k) to Human VTCN1 / B7-H4

Cat. Num. AAA51920

• Gene Names: VTCN1; B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Protien G purified from tissue culture supernatant
• Synonyms: VTCN1 / B7-H4, Antibody; Mouse Monoclonal [clone H74] (IgG1,k) to Human VTCN1 / B7-H4; Anti-VTCN1 / B7-H4 Antibody (clone H74) IHC-plus; VTCN1; B7 superfamily member 1; B7 Superfamily; member 1; B7h.5; B7S1; B7H4; PRO1291; VCTN1; RP11-229A19.4; B7 family member; H4; B7-H4; B7X; Protein B7S1; Human VTCN1; anti-VTCN1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: H74
• Purity/Purification: Protien G purified from tissue culture supernatant
• Form/Format: PBS with 0.09% (w/v) sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 282

• Applicable Applications for anti-VTCN1 antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
• Target Species: Human
• Immunogen Description: VTCN1/B7-H4 antibody was raised against human VTCN1
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 2-8 degree C

FCM/FACS (Flow Cytometry)

(Staining of non-transfected 293T cells with 0.125 ug of Purified Mouse IgG1, K isotype control (open histogram) or 0.125 ug of Purified anti-human B7-H4 (H74) (colored histogram) followed by Biotin Anti-Mouse IgG and SAv-PE. Total cells were used for analysis.)

FCM/FACS (Flow Cytometry)

(Staining of human B7-H4 transfected 293T cells with 0.125 ug of Purified Mouse IgG1, K isotype control (open histogram) or 0.125 ug of Purified anti-human B7-H4 (H74) (colored histogram) followed by Biotin Anti-Mouse IgG and SAv-PE. Total cells were used for analysis.)

IHC (Immunohiostchemistry)

(Anti-VTCN1 / B7-H4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)

IHC (Immunohistochemistry)

(Anti-VTCN1 / B7-H4 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 99028881
• NCBI GeneID: 79679
• NCBI Accession #: NP_078902.2
• NCBI GenBank Nucleotide #: NM_024626.3
• UniProt Accession #: Q7Z7D3; Q0GN76; Q45VN0; Q5WPZ3; Q6P097; Q9H6B2
• NCBI Official Full Name: V-set domain-containing T-cell activation inhibitor 1 isoform 1
• NCBI Official Synonym Full Names: V-set domain containing T cell activation inhibitor 1
• NCBI Official Symbol: VTCN1
• NCBI Official Synonym Symbols: B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291
• NCBI Protein Information: V-set domain-containing T-cell activation inhibitor 1; VTCN1; B7 homolog 4; B7 family member, H4; B7 superfamily member 1; T cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; immune costimulatory protein B7-H4
• UniProt Protein Name: V-set domain-containing T-cell activation inhibitor 1
• UniProt Gene Name: VTCN1
• UniProt Synonym Gene Names: B7-H4
• UniProt Entry Name: VTCN1_HUMAN