Bioactivity
B7 Homolog 4/B7-H4/VTCN1 Biotinylated Active Protein | B7-H4/VTCN1 active protein
Recombinant Human B7 Homolog 4/B7-H4/VTCN1 (C-Fc-Avi) Biotinylated
Gene Names
VTCN1; B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291; RP11-229A19.4
Purity
>95% as determined by reducing SDS-PAGE
Synonyms
B7 Homolog 4/B7-H4/VTCN1 Biotinylated; N/A; Recombinant Human B7 Homolog 4/B7-H4/VTCN1 (C-Fc-Avi) Biotinylated; B7S1; B7x; Vtcn1; B7h.5; B7-H4; B7H4T-cell costimulatory molecule B7x; B7S1VCTN1; B7XPRO1291; FLJ22418; Immune costimulatory protein B7-H4; Protein B7S1; T cell costimulatory molecule B7x; V-set domain containing T cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1; B7-H4/VTCN1 active protein
Host
Human Cells
Purity/Purification
>95% as determined by reducing SDS-PAGE
Form/Format
Lyophilized from a 0.2um filtered solution of PBS, pH7.4.
Sequence
Phe29-Ala258
Species
Human
Tag
C-Fc-Avi
Endotoxin
<1.0EU per ug as determined by the LAL method.
Protein Construction
Biotinylated Recombinant Human B7 Homolog 4 is produced by our Mammalian expression system and the target gene encoding Phe29-Ala258 is expressed with a Fc, Avi tag at the C-terminus.
Activity
Immobilized Anti-Human B7-H4 mAb at 2ug/ml (100ul/well) can bind Biotinylated Human B7-H4-Fc-Avi . The ED50 of Biotinylated Human B7-H4-Fc-Avi is 0.23ng/ml.
Preparation and Storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.
This product is provided as lyophilized powder which is shipped with ice packs.
This product is provided as lyophilized powder which is shipped with ice packs.
Bioactivity
Bioactivity
SDS-PAGE
SDS-PAGE
Related Product Information for B7-H4/VTCN1 active protein
B7 Homolog 4 (B7-H4) is glycosylated member of the B7 family of immune costimulatory proteins. Mature human B7-H4 consists of a 235 amino acid (aa) extracellular domain (ECD) with two Ig-like V-type domains, a 21 aa transmembrane segment, and a 2 aa cytoplasmic tail. It is widely expressed, including in kidney, liver, lung, pancreas, placenta, prostate, spleen, testis and thymus. B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. It also involved in promoting epithelial cell transformation.
Product Categories/Family for B7-H4/VTCN1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,878 Da
NCBI Official Full Name
B7-H4
NCBI Official Synonym Full Names
V-set domain containing T cell activation inhibitor 1
NCBI Official Symbol
VTCN1
NCBI Official Synonym Symbols
B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291; RP11-229A19.4
NCBI Protein Information
V-set domain-containing T-cell activation inhibitor 1; B7 family member, H4; B7 superfamily member 1; T cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; immune costimulatory protein B7-H4
UniProt Protein Name
V-set domain-containing T-cell activation inhibitor 1
UniProt Gene Name
VTCN1
UniProt Synonym Gene Names
B7H4
UniProt Entry Name
VTCN1_HUMAN
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Product Notes
The B7-H4/VTCN1 vtcn1 (Catalog #AAA177958) is an Active Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Phe29-Ala2 58. It is sometimes possible for the material contained within the vial of "B7 Homolog 4/B7-H4/VTCN1 Biotinylated, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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