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FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with Wilms Tumor Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit anti-Human, Mouse Wilms Tumor Protein Monoclonal Antibody | anti-WT antibody
Wilms Tumor Protein Antibody
Gene Names
WT1; GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
Affinity-chromatography
Synonyms
Wilms Tumor Protein, Antibody; Wilms Tumor Protein Antibody; WIT 2 antibody; WT 1 antibody; AWT1 antibody; FWT1 antibody; GUD antibody; NPHS4 antibody; WAGR antibody; Wilms tumor 1 antibody; Wilms Tumor antibody; Wilms tumor protein antibody; Wilms' tumor gene antibody; Wilms' tumor protein antibody; WIT2 antibody; WT antibody; WT1 antibody; WT1_HUMAN antibody; WT33 antibody; anti-WT antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
SC06-41
Purity/Purification
Affinity-chromatography
Form/Format
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Applicable Applications for anti-WT antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC)
Application Notes
WB: 1:1000-1:2000
IHC: 1:100-1:200
ICC/IF: 1:50-1:200
FC: 1:20-1:1000
IHC: 1:100-1:200
ICC/IF: 1:50-1:200
FC: 1:20-1:1000
Immunogen
A synthesized peptide derived from human WT1
Preparation and Storage
Store at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with Wilms Tumor Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with Wilms Tumor Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining Wilms Tumor Protein in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Wilms Tumor Protein in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Wilms Tumor Protein in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Wilms Tumor Protein in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Wilms Tumor Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Wilms Tumor Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse kidney, using Wilms Tumor Protein Antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse kidney, using Wilms Tumor Protein Antibody.)
WB (Western Blot)
(All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)
WB (Western Blot)
(All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)
WB (Western Blot)
(Western blot analysis of WT1 expression in K562 cell lysate.)
WB (Western Blot)
(Western blot analysis of WT1 expression in K562 cell lysate.)
WB (Western Blot)
(All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)
WB (Western Blot)
(All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)
Related Product Information for anti-WT antibody
Wilms' tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10, 000 infants and, like retinoblastoma, is observed in both sporadic and inherited forms. The Wilms' tumor locus has been mapped at chromosome 11p13 as a tumor suppressor gene which encodes a DNA binding protein with four zinc fingers and a glutamine-proline rich amino-terminus. The Wilms' tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators. However, in contrast to Egr transcription factors, WT1 behaves as a transcriptional repressor in transient transfection assays with synthetic promotor constructs.
Product Categories/Family for anti-WT antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 55kDa
NCBI Official Full Name
Wilms tumor protein isoform B
NCBI Official Synonym Full Names
Wilms tumor 1
NCBI Official Symbol
WT1
NCBI Official Synonym Symbols
GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1
NCBI Protein Information
Wilms tumor protein; amino-terminal domain of EWS|last three zinc fingers of the DNA-binding domain of WT1
UniProt Protein Name
Wilms tumor protein
UniProt Gene Name
WT1
UniProt Entry Name
WT1_HUMAN
Similar Products
Product Notes
The WT wt1 (Catalog #AAA30123) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Wilms Tumor Protein Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Wilms Tumor Protein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC). WB: 1:1000-1:2000 IHC: 1:100-1:200 ICC/IF: 1:50-1:200 FC: 1:20-1:1000. Researchers should empirically determine the suitability of the WT wt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Wilms Tumor Protein, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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