WB (Western Blot)
(Western blot analysis of lysates from A431, HepG2, mouse NIH/3T3, rat PC-12 and C6 cell line, mouse brain tissue (from left to right), using YWHAZ Antibody. AAA283483 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Mouse YWHAZ Monoclonal Antibody | anti-YWHAZ antibody
YWHAZ Antibody
Gene Names
YWHAZ; HEL4; YWHAD; KCIP-1; HEL-S-3; 14-3-3-zeta
Reactivity
Human, mouse, rat (Predicted Reactivity: Bovine,Sheep)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Purified Mouse Monoclonal Antibody (Mab)
Synonyms
YWHAZ, Antibody; YWHAZ Antibody; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1; YWHAZ; anti-YWHAZ antibody
Host
Mouse
Reactivity
Human, mouse, rat (Predicted Reactivity: Bovine,Sheep)
Clonality
Monoclonal
Isotype
IgG2b,kappa
Specificity
This YWHAZ antibody is generated from a mouse immunized with a recombinant protein from human YWHAZ.
Purity/Purification
Purified Mouse Monoclonal Antibody (Mab)
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Sequence Length
245
Applicable Applications for anti-YWHAZ antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Cellular location
Cytoplasm. Melanosome. Note=Located to stage I to stage IV melanosomes.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from A431, HepG2, mouse NIH/3T3, rat PC-12 and C6 cell line, mouse brain tissue (from left to right), using YWHAZ Antibody. AAA283483 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from A431, HepG2, mouse NIH/3T3, rat PC-12 and C6 cell line, mouse brain tissue (from left to right), using YWHAZ Antibody. AAA283483 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
IF (Immunofluorescence)
(Fluorescent image of U251 cells stained with YWHAZ Antibody. AAA283483 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
IF (Immunofluorescence)
(Fluorescent image of U251 cells stained with YWHAZ Antibody. AAA283483 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Related Product Information for anti-YWHAZ antibody
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Product Categories/Family for anti-YWHAZ antibody
References
Zupan L.A.,et al.J. Biol. Chem. 267:8707-8710(1992).
Seluja G.A.,et al.Biochim. Biophys. Acta 1395:281-287(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Gevaert K.,et al.Nat. Biotechnol. 21:566-569(2003).
Seluja G.A.,et al.Biochim. Biophys. Acta 1395:281-287(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Gevaert K.,et al.Nat. Biotechnol. 21:566-569(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
14-3-3 protein zeta/delta
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta
NCBI Official Symbol
YWHAZ
NCBI Official Synonym Symbols
HEL4; YWHAD; KCIP-1; HEL-S-3; 14-3-3-zeta
NCBI Protein Information
14-3-3 protein zeta/delta
UniProt Protein Name
14-3-3 protein zeta/delta
UniProt Gene Name
YWHAZ
UniProt Synonym Gene Names
KCIP-1
UniProt Entry Name
1433Z_HUMAN
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Product Notes
The YWHAZ ywhaz (Catalog #AAA283483) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The YWHAZ Antibody reacts with Human, mouse, rat (Predicted Reactivity: Bovine,Sheep) and may cross-react with other species as described in the data sheet. AAA Biotech's YWHAZ can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the YWHAZ ywhaz for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "YWHAZ, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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