ZEB1 recombinant antibody

Rabbit anti-ZEB1 Recombinant Monoclonal Antibody [BLR217K]

Cat. Num. AAA23870

• Gene Names: ZEB1; BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Purified
• Synonyms: ZEB1; Monoclonal Recombinant Antibody; Rabbit anti-ZEB1 Recombinant Monoclonal Antibody [BLR217K]; PPCD3; posterior polymorphous corneal dystrophy 3; zinc finger homeodomain enhancer-binding protein; zinc finger E-box-binding homeobox 1; ZFHX1A; ZFHEP; transcription factor 8 (represses interleukin 2 expression); Transcription factor 8; TCF-8; AREB6; NIL-2-A zinc finger protein; NIL2A; negative regulator of IL2; FECD6; DELTAEF1; delta-crystallin enhancer binding factor 1; BZP; TCF8; Zinc finger E-box-binding homeobox 1; zinc finger E-box binding homeobox 1; ZEB1 recombinant antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: BLR217K
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
• Concentration: 1000ug/ml (varies by lot)

• Applicable Applications for ZEB1 recombinant antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; IP: 6 ul/mg lysate; IHC: 1:100 to 1:500. Epitope retrieval with citrate Buffer pH 6.0 is recommended for FFPE tissue sections.; ICC: 1:100 to 1:500. Epitope retrieval with citrate Buffer pH 6.0 is recommended for FFPE cell sections.; FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.25 ul per 1 x 10^6 cells.
• Immunogen: Between 750 and 800
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human ZEB1 by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, HeLa, RKO, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ZEB1 recombinant monoclonal antibody (AAA23870 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

WB (Western Blot)

(Detection of mouse ZEB1 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ZEB1 recombinant monoclonal antibody (AAA23870 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)

IP (Immunoprecipitation)

(Detection of human ZEB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-ZEB1 recombinant monoclonal antibody (AAA23870 lot 1) used for IP at 6 ul/mg lysate. ZEB1 was also immunoprecipitated by a second antibody against a different epitope of ZEB1 . For blotting immunoprecipitated ZEB1, AAA23870 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IHC (Immunohistchemistry)

(Detection of human ZEB1 by immunohistochemistry. Sample: FFPE section of glioblastoma. Antibody: Rabbit anti-ZEB1 recombinant monoclonal antibody (AAA23870-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry)

(Detection of mouse ZEB1 by immunohistochemistry. Sample: FFPE section of mouse Renca tumor. Antibody: Rabbit anti-ZEB1 recombinant monoclonal antibody (AAA23870-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry)

(Detection of human ZEB1 by immunocytochemistry. Sample: FFPE section of RKO cells. Antibody: Rabbit anti-ZEB1 recombinant monoclonal antibody (AAA23870-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry)

(Detection of mouse ZEB1 by immunocytochemistry. Sample: FFPE section of ND7/23 cells. Antibody: Rabbit anti-ZEB1 recombinant monoclonal antibody (AAA23870-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry)

(Detection of mouse Zeb1 (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti-Zeb1 recombinant monoclonal (AAA23870) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry)

(Detection of human Zeb1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-Zeb1 recombinant monoclonal (AAA23870) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

Related Product Information for ZEB1 recombinant antibody

ZEB1 (zinc-finger E-box-binding homeobox 1), also known as TCF8 (transcription factor 8), is a delta-EF1/ZFH-1 C2H2-type zinc finger that bears seven C2H2-type zinc fingers and one homeobox DNA-binding domain. ZEB1 is a transcriptional repressor of interleukin-2 (IL-2). Mutations in the ZEB1 gene are associated with posterior corneal dystrophy type 3 (PPC3) a rare disorder characterized by metaplasia and overgrowth of corneal endothelial cells. ZEB1 appears to be critical to epithelial to mesenchymal transitions (EMT) during development and cancer metastasis. As a transcription factor involved in EMT, ZEB1 promotes EMT by repressing genes associated with the epithelial phenotype and activates genes associated with the mesenchymal phenotype.

Product Categories/Family for ZEB1 recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 291575186
• NCBI GeneID: 6935
• NCBI Accession #: NP_110378.3
• NCBI GenBank Nucleotide #: NM_030751
• UniProt Accession #: P37275; Q12924; Q13800; Q2KJ05; Q5T968; Q5VZ84; Q8NB68; B4DJV0; B4DUW9; E9PCM7; F5H4I8
• Molecular Weight: 122,084 Da
• NCBI Official Full Name: Homo sapiens zinc finger E-box binding homeobox 1 (ZEB1), transcript variant 2, mRNA
• NCBI Official Synonym Full Names: zinc finger E-box binding homeobox 1
• NCBI Official Symbol: ZEB1
• NCBI Official Synonym Symbols: BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1
• NCBI Protein Information: zinc finger E-box-binding homeobox 1; delta-crystallin enhancer binding factor 1; negative regulator of IL2; posterior polymorphous corneal dystrophy 3; transcription factor 8 (represses interleukin 2 expression); zinc finger homeodomain enhancer-binding
• UniProt Protein Name: Zinc finger E-box-binding homeobox 1
• UniProt Gene Name: ZEB1
• UniProt Synonym Gene Names: AREB6; TCF8; TCF-8
• UniProt Entry Name: ZEB1_HUMAN