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Application Data
Interleukin 18 (IL18) Active Protein | IL18 active protein
Active Interleukin 18 (IL18)
Gene Names
Il18; Igif; Il-18
Reactivity
Mouse
Applications
Activity Assay, Cell Culture
Purity
> 90%
Synonyms
Interleukin 18 (IL18); N/A; Active Interleukin 18 (IL18); IGIF; IL-1g; IL1F4; IL1-F4; Interferon-Gamma-Inducing Factor; Interleukin-1 Family Member 4; Iboctadekin; Interleukin-1 Gamma; IL18 active protein
Reactivity
Mouse
Purity/Purification
> 90%
Form/Format
Freeze-dried powder
Sequence Length
192
Applicable Applications for IL18 active protein
Activity Assay, Cell Culture
Tag
N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Fragment
Asn36~Ser192
Isoelectric Point
5
Application Data
Application Data
Application Data
Application Data
SDS-PAGE
SDS-PAGE
Activity
(IL18 (interleukin 18) is a cytokine that shares similarities in structure with IL1, and is produced by macrophages and other cells. IL18 has been reported to stimulate T cells proliferation and NK cell activity, besides, murine IL18 has been found to enchance the production of INFgamma in spleen cells in vitro. Thus, a stimulation assay was conducted to detect the activity of IL18 using murine spleen cells. Briefly, murine spleen cells were seeded into wells of 6-well plates at a density of 2×106 cells/mL in RPMI-1640 with the addition of various concentrations of IL18. After incubation for 48 hours, the concentration of INFgamma in the cell supernatant was detected using an ELISA kit. INFgamma levels in the cell supernatant of spleen cells increased significantly after stimulated with IL18, the data was shown in Figure 1.)
Activity
(IL18 (interleukin 18) is a cytokine that shares similarities in structure with IL1, and is produced by macrophages and other cells. IL18 has been reported to stimulate T cells proliferation and NK cell activity, besides, murine IL18 has been found to enchance the production of INFgamma in spleen cells in vitro. Thus, a stimulation assay was conducted to detect the activity of IL18 using murine spleen cells. Briefly, murine spleen cells were seeded into wells of 6-well plates at a density of 2×106 cells/mL in RPMI-1640 with the addition of various concentrations of IL18. After incubation for 48 hours, the concentration of INFgamma in the cell supernatant was detected using an ELISA kit. INFgamma levels in the cell supernatant of spleen cells increased significantly after stimulated with IL18, the data was shown in Figure 1.)
Product Categories/Family for IL18 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Weight: 19.4kDa
Accurate Molecular Weight: 19kDa
Accurate Molecular Weight: 19kDa
NCBI Official Full Name
interleukin-18 isoform a
NCBI Official Synonym Full Names
interleukin 18
NCBI Official Symbol
Il18
NCBI Official Synonym Symbols
Igif; Il-18
NCBI Protein Information
interleukin-18
UniProt Protein Name
Interleukin-18
UniProt Gene Name
Il18
UniProt Synonym Gene Names
Igif; IL-18; IFN-gamma-inducing factor; IL-1 gamma
Similar Products
Product Notes
The IL18 il18 (Catalog #AAA148130) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. The Active Interleukin 18 (IL18) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 18 (IL18) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the IL18 il18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 18 (IL18), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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