Mouse KC CLIA Kit | KC clia kit

KC (Murine) LumiAb ELISA Kit

Cat. Num. AAA318575

• Gene Names: Cxcl1; KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1
• Reactivity: Mouse
• Synonyms: KC; N/A; KC (Murine) LumiAb ELISA Kit; Murine KC; Growth-regulated alpha protein; C-X-C motif chemLumine 1; Platelet-derived growth factor-inducible protein KC; Secretory protein N51; KC clia kit

• Reactivity: Mouse
• Specificity: The LumiAb Murine JE/MCP-1 ELISA is capable of recognizing both recombinant and naturally produced Murine JE/MCP-1 proteins. The antigens listed below were tested at 50 ng/ml and exhibited 100% cross reactivity.
• Sequence Length: 96
• Assay Type: Quantitative Sandwich
• Sensitivity: 15 pg/ml.

• Preparation and Storage: Store at 4 degree C for 6 months.

Standard Curve (Sample)

Related Product Information for KC clia kit

Principle of the Assay: The LumiAb Murine JE/MCP-1 Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Murine JE/MCP-1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Murine JE/MCP-1 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Murine JE/MCP-1 or Monocyte Chemotactic Protein 1, mainly known as C-C Motif Chemokine 2, is a 148 amino acid cytokine protein expressed from chromosome 11. After initial synthesis and translocation, the 23 residue signal sequence is cleaved, allowing the 125 residue JE/MCP-1 peptide to properly fold and mature. Belonging to the intercrine beta (chemokine C-C) family, the monomeric and homodimeric JE/MCP-1 proteins function as chemotactic factors that attract monocytes, but not neutrophils. Furthermore, they are typically induced by platelet-derived growth factors (PDGF).

Product Categories/Family for KC clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 6680109
• NCBI GeneID: 14825
• NCBI Accession #: NP_032202.1
• NCBI GenBank Nucleotide #: NM_008176.3
• UniProt Accession #: P12850
• NCBI Official Full Name: growth-regulated alpha protein
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 1
• NCBI Official Symbol: Cxcl1
• NCBI Official Synonym Symbols: KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1
• NCBI Protein Information: growth-regulated alpha protein
• UniProt Protein Name: Growth-regulated alpha protein
• UniProt Gene Name: Cxcl1
• UniProt Synonym Gene Names: Gro; Gro1; Mgsa; Scyb1; HSF
• UniProt Entry Name: GROA_MOUSE