Mouse KC ELISA Kit | KC elisa kit

KC (Murine) OmniKine ELISA Kit

Cat. Num. AAA315679

• Gene Names: Cxcl1; KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1
• Reactivity: Mouse
• Synonyms: KC; N/A; KC (Murine) OmniKine ELISA Kit; Growth-regulated alpha protein; C-X-C motif chemokine 1; Platelet-derived growth factor-inducible protein KC; Secretory protein N51; KC elisa kit

• Reactivity: Mouse
• Specificity: The OmniKine™ Murine KC ELISA is capable of recognizing both recombinant and naturally produced Murine KC proteins. The antigens listed below were tested at 50 ng/ml the following antigens exhibited less than 1% cross reactivity Rat: GROalpha The antigens listed below were tested at 50 ng/ml the following antigens exhibited less than 1% cross reactivity Human ENA-78, GCP-2, GROalph, GRObeta, GROgamma, IL-8, NAP-2 Murine IL-13 Rat GRObeta.
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 96
• Assay Type: Quantitative Sandwich
• Samples: Supernatants, Sera and Plasma
• Sensitivity: 16-1000 pg/ml

• Detection Method: Colorimetric 450 nm
• Subtype: None
• Preparation and Storage: Store entire kit at 4 degree C until use.

Application Data

(The OmniKine Murine KC ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Murine KC proteins within the range of 8-1000 pg/ml.)

Related Product Information for KC elisa kit

Background: Murine KC or Growth-regulated Alpha Protein, also known commonly as Chemokine (C-X-C Motif) Ligand 1, is a 96 amino acid cytokine protein encoded by the Cxcl1 gene located at 5 E-F locus on chromosome 5. After translation of the protein, the resulting peptide is proteolytically cleaved into the 24 residue signal sequence as well as the 72 residue Growth-regulated Alpha Protein. However, if the first 4 residues are cleaved from the mature Growth-regulated Alpha Protein, the resulting peptide is known commonly as KC (5-72) or Hematopoietic Synergistic Factor. The N-terminal processed form KC (5-72) is produced by proteolytic cleavage after secretion from bone marrow stromal cells. Moreover, Murine Growth-regulated Alpha Protein has chemotactic activity for neutrophils and contributes to their activation during inflammation. Moreover, it is known to be a hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. On the other hand, its shorter derivative, KC (5-72) is commonly believed to show greater and more enhanced hematopoietic activity. The induction of KC is typically driven by Platelet-Derived Growth Factor (PDGF), but in the lungs, induction is attributed to lipopolysaccharides from bacterial cell walls or inflammation.

Principle of the Assay: The OmniKine™ Murine KC ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Murine KC concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human SDF-1alpha while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.

NCBI and Uniprot Product Information

• NCBI GI #: 6680109
• NCBI GeneID: 14825
• NCBI Accession #: NP_032202.1
• NCBI GenBank Nucleotide #: NM_008176.3
• UniProt Accession #: P12850
• Molecular Weight: 10,254 Da
• NCBI Official Full Name: growth-regulated alpha protein
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 1
• NCBI Official Symbol: Cxcl1
• NCBI Official Synonym Symbols: KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1
• NCBI Protein Information: growth-regulated alpha protein
• UniProt Protein Name: Growth-regulated alpha protein
• UniProt Gene Name: Cxcl1
• UniProt Synonym Gene Names: Gro; Gro1; Mgsa; Scyb1; HSF