Standard Curve (Sample)
(Fig.1. Mouse Adiponectin (ADP) Standard Curve.)
Mouse Adiponectin (ADP) ELISA Kit | ADP elisa kit
Mouse Adiponectin (ADP) ELISA Kit
Gene Names
Adipoq; Ad; APN; Acdc; Adid; apM1; 30kDa; GBP28; adipo; Acrp30
Reactivity
Mouse
Synonyms
Adiponectin (ADP); N/A; Mouse Adiponectin (ADP) ELISA Kit; ADP; ACDC; ACRP30; ADIPQTL1; ADPN; APM-1; APM1; GBP28; adiponectin; OTTHUMP00000210047; OTTHUMP00000210048; adipocyte; C1Q and collagen domain containing; C1Q and collagen domain-containing; adipo; ADP elisa kit
Reactivity
Mouse
Specificity
Mouse Adiponectin (ADP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ADP. No significant cross-reactivity or interference between Mouse ADP and analogues was observed.
Sequence Length
247
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Mouse Adiponectin (ADP) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Mouse Adiponectin (ADP) Standard Curve.)
Related Product Information for ADP elisa kit
Description: This Mouse Adiponectin (ADP) ELISA Kit employs a two-site sandwich ELISA to quantitate ADP in samples. An antibody specific for ADP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Adiponectin, alternatively named Adipocyte Complement-Related Protein of 30 kDa, shares structural similarity with complement factor C1q and is a member of the family of defense collagens. It is secreted exclusively by differentiated adipocytes and circulates at high concentrations. Adiponectin has a modular structure comprising an N-terminal collagenous domain with multiple collagen triple helix repeats, followed by a C-terminal C1q-like globular domain. The globular domain has similar folding topology with tumor necrosis factor-alpha and assembles into homotrimers. Higher order oligomeric adiponectins are also formed via interactions between the collagenous stalk. A truncated form of Adiponectin containing only the globular domain can be generated by proteolytic cleavage.
Background: Adiponectin, alternatively named Adipocyte Complement-Related Protein of 30 kDa, shares structural similarity with complement factor C1q and is a member of the family of defense collagens. It is secreted exclusively by differentiated adipocytes and circulates at high concentrations. Adiponectin has a modular structure comprising an N-terminal collagenous domain with multiple collagen triple helix repeats, followed by a C-terminal C1q-like globular domain. The globular domain has similar folding topology with tumor necrosis factor-alpha and assembles into homotrimers. Higher order oligomeric adiponectins are also formed via interactions between the collagenous stalk. A truncated form of Adiponectin containing only the globular domain can be generated by proteolytic cleavage.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
adiponectin
NCBI Official Synonym Full Names
adiponectin, C1Q and collagen domain containing
NCBI Official Symbol
Adipoq
NCBI Official Synonym Symbols
Ad; APN; Acdc; Adid; apM1; 30kDa; GBP28; adipo; Acrp30
NCBI Protein Information
adiponectin
UniProt Protein Name
Adiponectin
UniProt Gene Name
Adipoq
UniProt Synonym Gene Names
Acdc; Acrp30; Apm1; ACRP30
UniProt Entry Name
ADIPO_MOUSE
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Product Notes
The Mouse ADP adipoq (Catalog #AAA332397) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332397 ELISA Kit recognizes Mouse ADP. It is sometimes possible for the material contained within the vial of "Adiponectin (ADP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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