Mouse Ankyrin repeat domain-containing protein 35 (ANKRD35) ELISA Kit | ANKRD35 elisa kit

Mouse Ankyrin repeat domain-containing protein 35 (ANKRD35) ELISA Kit

Cat. Num. AAA244287

• Reactivity: Mouse
• Synonyms: Ankyrin repeat domain-containing protein 35 (ANKRD35); N/A; Mouse Ankyrin repeat domain-containing protein 35 (ANKRD35) ELISA Kit; ANKRD35 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of alphaMSH-Ab. No significant cross-reactivity or interference between alphaMSH-Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between alphaMSH-Ab and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for ANKRD35 elisa kit

Principle of the Assay: alphaMSH-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizingalphaMSH-Ab antigen and an alphaMSH-Ab-HRP conjugate. The assay sample and buffer are incubated together with alphaMSH-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the alphaMSH-Ab concentration since alphaMSH-Ab from samples and alphaMSH-Ab-HRP conjugate compete for thealphaMSH antigen binding site. Since the number of sites is limited, as more sites are occupied by alphaMSH-Ab from the sample, fewer sites are left to bind alphaMSH-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The alphaMSH-Ab concentration in each sample is interpolated from this standard curve.

Intended Uses: This alphaMSH-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse alphaMSH-Ab. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for ANKRD35 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 526118249
• NCBI GeneID: 148741
• NCBI Accession #: NP_653299.4
• NCBI GenBank Nucleotide #: NM_144698.4
• Molecular Weight: 109,966 Da
• NCBI Official Full Name: ankyrin repeat domain-containing protein 35 isoform 1
• NCBI Official Synonym Full Names: ankyrin repeat domain 35
• NCBI Official Symbol: ANKRD35
• NCBI Protein Information: ankyrin repeat domain-containing protein 35
• UniProt Protein Name: Ankyrin repeat domain-containing protein 35
• UniProt Gene Name: ANKRD35
• UniProt Entry Name: ANR35_HUMAN