Mouse Ankyrin repeat domain-containing protein 36A (ANKRD36) ELISA Kit | ANKRD36 elisa kit

Mouse Ankyrin repeat domain-containing protein 36A (ANKRD36) ELISA Kit

Cat. Num. AAA245201

• Gene Names: ANKRD36; UNQ2430
• Reactivity: Mouse
• Synonyms: Ankyrin repeat domain-containing protein 36A (ANKRD36); N/A; Mouse Ankyrin repeat domain-containing protein 36A (ANKRD36) ELISA Kit; ANKRD36 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of ANKRD36. No significant cross-reactivity or interference between ANKRD36 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANKRD36 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for ANKRD36 elisa kit

Principle of the Assay: ANKRD36 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ANKRD36 antibody and an ANKRD36-HRP conjugate. The assay sample and buffer are incubated together with ANKRD36-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANKRD36 concentration since ANKRD36 from samples and ANKRD36-HRP conjugate compete for the anti-ANKRD36 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANKRD36 from the sample, fewer sites are left to bind ANKRD36-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANKRD36 concentration in each sample is interpolated from this standard curve.

Intended Uses: This ANKRD36 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ANKRD36. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for ANKRD36 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 256222280
• NCBI GeneID: 375248
• NCBI Accession #: NP_001157787.1
• NCBI GenBank Nucleotide #: NM_001164315.1
• Molecular Weight: 19,755 Da
• NCBI Official Full Name: ankyrin repeat domain-containing protein 36A
• NCBI Official Synonym Full Names: ankyrin repeat domain 36
• NCBI Official Symbol: ANKRD36
• NCBI Official Synonym Symbols: UNQ2430
• NCBI Protein Information: ankyrin repeat domain-containing protein 36A; ankyrin-related
• UniProt Protein Name: Ankyrin repeat domain-containing protein 36A
• UniProt Gene Name: ANKRD36
• UniProt Synonym Gene Names: ANKRD36A; KIAA1641; UNQ2430/PRO499
• UniProt Entry Name: AN36A_HUMAN