Mouse Bisphosphoglycee mutase (BPGM) ELISA Kit | BPGM elisa kit

Mouse Bisphosphoglycee mutase (BPGM) ELISA Kit

Cat. Num. AAA27110

• Gene Names: BPGM; DPGM
• Reactivity: Mouse
• Synonyms: Bisphosphoglycee mutase (BPGM); N/A; Mouse Bisphosphoglycee mutase (BPGM) ELISA Kit; BPGM elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of BPGM. No significant cross-reactivity or interference between BPGM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BPGM and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for BPGM elisa kit

Principle of the Assay: BPGM ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BPGM antibody and an BPGM-HRP conjugate. The assay sample and buffer are incubated together with BPGM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BPGM concentration since BPGM from samples and BPGM-HRP conjugate compete for the anti-BPGM antibody binding site. Since the number of sites is limited, as more sites are occupied by BPGM from the sample, fewer sites are left to bind BPGM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BPGM concentration in each sample is interpolated from this standard curve.

Intended Uses: This BPGM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BPGM.. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

NCBI and Uniprot Product Information

• NCBI GI #: 641386940
• NCBI GeneID: 669
• NCBI Accession #: NP_001280014.1
• NCBI GenBank Nucleotide #: NM_001293085.1
• Molecular Weight: 30,005 Da
• NCBI Official Full Name: bisphosphoglycerate mutase
• NCBI Official Synonym Full Names: 2,3-bisphosphoglycerate mutase
• NCBI Official Symbol: BPGM
• NCBI Official Synonym Symbols: DPGM
• NCBI Protein Information: bisphosphoglycerate mutase; BPG-dependent PGAM; 2,3-diphosphoglycerate mutase; 2,3-bisphosphoglycerate synthase; erythrocyte 2,3-bisphosphoglycerate mutase; 2,3-bisphosphoglycerate mutase, erythrocyte
• UniProt Protein Name: Bisphosphoglycerate mutase
• UniProt Gene Name: BPGM
• UniProt Synonym Gene Names: BPGM; DPGM
• UniProt Entry Name: PMGE_HUMAN