Rat Bisphosphoglycee mutase (BPGM) ELISA Kit | BPGM elisa kit
Rat Bisphosphoglycee mutase (BPGM) ELISA Kit
Gene Names
BPGM; DPGM
Reactivity
Rat
Synonyms
Bisphosphoglycee mutase (BPGM); N/A; Rat Bisphosphoglycee mutase (BPGM) ELISA Kit; BPGM elisa kit
Reactivity
Rat
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for BPGM elisa kit
Intended Uses: This BP180-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine BP180-Ab. This ELISA kit for research use only!
Principle of the Assay: BP180-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing a BP180 antigen and an BP180-Ab-HRP conjugate. The assay sample and buffer are incubated together with BP180-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BP180-Ab concentration since BP180-Ab from samples and BP180-Ab-HRP conjugate compete for the BP180 antigen binding site. Since the number of sites is limited, as more sites are occupied by BP180-Ab from the sample, fewer sites are left to bind BP180-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BP180-Ab concentration in each sample is interpolated from this standard curve.
Principle of the Assay: BP180-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing a BP180 antigen and an BP180-Ab-HRP conjugate. The assay sample and buffer are incubated together with BP180-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BP180-Ab concentration since BP180-Ab from samples and BP180-Ab-HRP conjugate compete for the BP180 antigen binding site. Since the number of sites is limited, as more sites are occupied by BP180-Ab from the sample, fewer sites are left to bind BP180-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BP180-Ab concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,005 Da
NCBI Official Full Name
bisphosphoglycerate mutase
NCBI Official Synonym Full Names
2,3-bisphosphoglycerate mutase
NCBI Official Symbol
BPGM
NCBI Official Synonym Symbols
DPGM
NCBI Protein Information
bisphosphoglycerate mutase; BPG-dependent PGAM; 2,3-diphosphoglycerate mutase; 2,3-bisphosphoglycerate synthase; erythrocyte 2,3-bisphosphoglycerate mutase; 2,3-bisphosphoglycerate mutase, erythrocyte
UniProt Protein Name
Bisphosphoglycerate mutase
UniProt Gene Name
BPGM
UniProt Synonym Gene Names
BPGM; DPGM
UniProt Entry Name
PMGE_HUMAN
