Mouse BMP4 ELISA Kit | BMP4 elisa kit

Mouse BMP4 ELISA Kit

Cat. Num. AAA263103

• Gene Names: Bmp4; Bmp-4; Bmp2b; Bmp2b1; Bmp2b-1
• Reactivity: Mouse
• Synonyms: BMP4; N/A; Mouse BMP4 ELISA Kit; BMP2B; DVR4; Bone morphogenetic protein 4; BMP-4; Bone morphogenetic protein 2B; BMP-2B; BMP4 elisa kit

• Reactivity: Mouse
• Specificity: The Mouse BMP4 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse BMP4 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
• Sequence Length: 408
• Samples: Bone Tissue, Cell Culture Supernates, Serum
• Sensitivity: <10 pg/ml

• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Standard Curve (Sample)

Related Product Information for BMP4 elisa kit

Principle of the Assay: The AAA Biotech' Mouse BMP4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse BMP4 in Bone Tissue, Cell Culture Supernates, Serum. This assay employs an antibody specific for Mouse BMP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse BMP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.!!Background/Introduction: Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23. The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-beta superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. The standard product used in this kit is recombinant BMP-4 with the molecular mass of 26KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 939320322
• NCBI GeneID: 12159
• NCBI Accession #: NP_001303289.1
• NCBI GenBank Nucleotide #: NM_001316360.1
• UniProt Accession #: P21275
• Molecular Weight: 46,497 Da
• NCBI Official Full Name: bone morphogenetic protein 4 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 4
• NCBI Official Symbol: Bmp4
• NCBI Official Synonym Symbols: Bmp-4; Bmp2b; Bmp2b1; Bmp2b-1
• NCBI Protein Information: bone morphogenetic protein 4
• UniProt Protein Name: Bone morphogenetic protein 4
• UniProt Gene Name: Bmp4
• UniProt Synonym Gene Names: Bmp-4; Dvr-4; BMP-4; BMP-2B
• UniProt Entry Name: BMP4_MOUSE