Mouse Brain Natriuretic Peptide (BNP) ELISA Kit | BNP elisa kit
Mouse Brain Natriuretic Peptide (BNP) ELISA Kit
Reactivity
Mouse
Synonyms
Brain Natriuretic Peptide (BNP); N/A; Mouse Brain Natriuretic Peptide (BNP) ELISA Kit; BNP elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of BNP. No significant cross-reactivity or interference between BNP and analogues was observed.
Sequence Length
1
Assay Type
Quantitative Competitive
Samples
Mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
12.35-1,000pg/mL
Sensitivity
<4.81pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BNP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BNP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for BNP elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to BNP has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled BNP and unlabeled BNP (Standards or samples) with the pre-coated antibody specific to BNP. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of BNP in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of BNP in the sample.
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of BNP in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of BNP in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Product Categories/Family for BNP elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
brain natriuretic peptide, partial
