Standard Curve (Sample)
Mouse CD70 ELISA Kit | CD70 elisa kit
Mouse CD70 ELISA Kit
Gene Names
Cd70; Cd27l; CD27LG; Tnfsf7; Tnlg8a
Reactivity
Mouse
Applications
ELISA
Synonyms
CD70; N/A; Mouse CD70 ELISA Kit; CD27L; CD27LG; TNFSF7; CD70 antigen; CD27 ligand; CD27-L; Tumor necrosis factor ligand superfamily member 7; CD70 elisa kit
Reactivity
Mouse
Specificity
The Mouse CD70 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CD70 proteins within the range of 31.2 pg/ml - 2000 pg/ml.
Sequence Length
195
Samples
Cell Culture Supernatants, Serum, Plasma.
Sensitivity
10 pg/ml.
Applicable Applications for CD70 elisa kit
ELISA (Sandwich ELISA)
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CD70 elisa kit
Principle of the Assay||The Mouse CD70 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD70 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CD70 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD70 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD70 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: CD70 (Cluster of Differentiation 70), also known as TNFSF7 or CD27L, is a ligand for CD27. This gene is mapped to 19p13.3. CD70 is a surface antigen found on activated, but not resting, T and B lymphocytes. It has been found that CD70 induced proliferation of costimulated T cells and enhanced generation of cytolytic T cells. CD70 is homologous to the ligands of the TNF receptor family, including TNF-alpha, TNF-beta, and the CD40 ligand, showing 19 to 24% amino acid sequence identity in the extracellular region.
Background/Introduction: CD70 (Cluster of Differentiation 70), also known as TNFSF7 or CD27L, is a ligand for CD27. This gene is mapped to 19p13.3. CD70 is a surface antigen found on activated, but not resting, T and B lymphocytes. It has been found that CD70 induced proliferation of costimulated T cells and enhanced generation of cytolytic T cells. CD70 is homologous to the ligands of the TNF receptor family, including TNF-alpha, TNF-beta, and the CD40 ligand, showing 19 to 24% amino acid sequence identity in the extracellular region.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
21,919 Da
NCBI Official Full Name
CD70 antigen
NCBI Official Synonym Full Names
CD70 antigen
NCBI Official Symbol
Cd70
NCBI Official Synonym Symbols
Cd27l; CD27LG; Tnfsf7; Tnlg8a
NCBI Protein Information
CD70 antigen
UniProt Protein Name
CD70 antigen
UniProt Gene Name
Cd70
UniProt Synonym Gene Names
Cd27l; Cd27lg; Tnfsf7; CD27-L
UniProt Entry Name
CD70_MOUSE
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Product Notes
The Mouse CD70 cd70 (Catalog #AAA261712) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA261712 ELISA Kit recognizes Mouse CD70. AAA Biotech's CD70 can be used in a range of immunoassay formats including, but not limited to, ELISA (Sandwich ELISA). Researchers should empirically determine the suitability of the CD70 cd70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD70, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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