Mouse Charcot Leyden Crystal Protein (CLC) ELISA Kit | CLC elisa kit

Mouse Charcot Leyden Crystal Protein (CLC) ELISA Kit

Cat. Num. AAA66444

• Gene Names: Clc; Clcp; gal-10
• Reactivity: Mouse
• Synonyms: Charcot Leyden Crystal Protein (CLC); N/A; Mouse Charcot Leyden Crystal Protein (CLC) ELISA Kit; LGALS10; LPPL; Eosinophil Lysophospholipase; Lysolecithin Acylhydrolase; Galectin 10; CLC elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of CLC. No significant cross-reactivity or interference between CLC and analogues was observed.
• Sequence Length: 68
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.044ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CLC were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CLC were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Standard Curve (Sample)

Related Product Information for CLC elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CLC in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CLC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CLC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CLC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CLC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 2829838
• NCBI GeneID: 12735
• UniProt Accession #: P97400
• Molecular Weight: 8,037 Da
• NCBI Official Full Name: Galectin-10
• NCBI Official Synonym Full Names: Charcot-Leyden crystal protein
• NCBI Official Symbol: Clc
• NCBI Official Synonym Symbols: Clcp; gal-10
• NCBI Protein Information: galectin-10
• UniProt Protein Name: Galectin-10
• UniProt Gene Name: Clc
• UniProt Synonym Gene Names: Clcp; Gal-10; CLC