Standard Curve (Sample)
(Mouse Chemerin/RARRES2 PicoKine ELISA Kit standard curve)
Mouse Chemerin/RARRES2 ELISA Kit | RARRES2 elisa kit
Mouse Chemerin/RARRES2 PicoKine ELISA Kit
Gene Names
Rarres2; AI303516; 0610007L05Rik
Reactivity
Mouse
Synonyms
Chemerin/RARRES2; N/A; Mouse Chemerin/RARRES2 PicoKine ELISA Kit; Retinoic acid receptor responder protein 2; retinoic acid receptor responder (tazarotene induced) 2; HP10433; RAR responsive protein TIG2; RAR-responsive protein TIG2; RARR2_HUMAN; RARRES2; Retinoic acid receptor responder (tazarotene induced) 2; Tazarotene induced gene 2 protein; Tazarotene-induced gene 2 protein; TIG2; RARRES2 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse Chemerin
Sequence Length
162
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
0.78ng/ml-50ng/ml
Sensitivity
< 20pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Mouse Chemerin/RARRES2 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Mouse Chemerin/RARRES2 PicoKine ELISA Kit standard curve)
Related Product Information for RARRES2 elisa kit
Principle of the assay: mouse Chemerin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Chemerin has been precoated onto 96-well plates. Standards(E.coli, T20-K162) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Chemerin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Chemerin amount of sample captured in plate.
Background: Chemerin, also known as RARRES2 or TIG2, is a protein that in humans is encoded by the RARRES2 gene. It is mapped to 7q36.1. Chemerin is a potent chemoattractant specific for antigen-presenting cells that requires proteolytic activation and acts as a ligand for the G protein-coupled receptor CMKLR1(also known as ChemR23). It is a 14 kDa protein secreted in an inactive form as prochemerin and is activated through cleavage of the C-terminus by inflammatory and coagulation serine proteases. Chemerin was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. What's more, the active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein.
Background: Chemerin, also known as RARRES2 or TIG2, is a protein that in humans is encoded by the RARRES2 gene. It is mapped to 7q36.1. Chemerin is a potent chemoattractant specific for antigen-presenting cells that requires proteolytic activation and acts as a ligand for the G protein-coupled receptor CMKLR1(also known as ChemR23). It is a 14 kDa protein secreted in an inactive form as prochemerin and is activated through cleavage of the C-terminus by inflammatory and coagulation serine proteases. Chemerin was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. What's more, the active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
18,350 Da
NCBI Official Full Name
Retinoic acid receptor responder protein 2
NCBI Official Synonym Full Names
retinoic acid receptor responder (tazarotene induced) 2
NCBI Official Symbol
Rarres2
NCBI Official Synonym Symbols
AI303516; 0610007L05Rik
NCBI Protein Information
retinoic acid receptor responder protein 2; chemerin
UniProt Protein Name
Retinoic acid receptor responder protein 2
UniProt Gene Name
Rarres2
UniProt Entry Name
RARR2_MOUSE
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Product Notes
The Mouse RARRES2 rarres2 (Catalog #AAA45806) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45806 ELISA Kit recognizes Mouse RARRES2. It is sometimes possible for the material contained within the vial of "Chemerin/RARRES2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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