Standard Curve (Sample)
Mouse Choline Acetyltransferase (ChAT) ELISA Kit | ChAT elisa kit
Mouse Choline Acetyltransferase (ChAT) ELISA Kit
Gene Names
Chat; B230380D24Rik
Reactivity
Mouse
Synonyms
Choline Acetyltransferase (ChAT); N/A; Mouse Choline Acetyltransferase (ChAT) ELISA Kit; CMS1A; CMS1A2; CHOACTase; Choline acetylase; ChAT elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of ChAT. No significant cross-reactivity or interference between ChAT and analogues was observed.
Sequence Length
645
Samples
Tissue homogenates, cell lysates and other biological fluids.
Assay Type
Sandwich
Detection Range
1.56-100ng/mL
Sensitivity
0.53ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ChAT were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ChAT were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ChAT elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ChAT in mouse tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ChAT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ChAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ChAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ChAT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ChAT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ChAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ChAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ChAT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72,339 Da
NCBI Official Full Name
choline O-acetyltransferase
NCBI Official Synonym Full Names
choline acetyltransferase
NCBI Official Symbol
Chat
NCBI Official Synonym Symbols
B230380D24Rik
NCBI Protein Information
choline O-acetyltransferase
UniProt Protein Name
Choline O-acetyltransferase
UniProt Gene Name
Chat
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Product Notes
The Mouse ChAT chat (Catalog #AAA63562) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA63562 ELISA Kit recognizes Mouse ChAT. It is sometimes possible for the material contained within the vial of "Choline Acetyltransferase (ChAT), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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