Application Data
Goat ChAT (Choline Acetyltransferase) Polyclonal Antibody | anti-CHAT antibody
Anti-Choline Acetyltransferase
Gene Names
CHAT; CMS1A; CMS1A2; CHOACTASE
Reactivity
Avian, Chicken, Guinea Pig, Human, Mouse, non-Human primates
Applications
Western Blot
Purity
Affinity Purified (Prepared from goat serum by affinity purification using a column to which the protein immunogen was coupled.)
Synonyms
ChAT (Choline Acetyltransferase), Antibody; Anti-Choline Acetyltransferase; anti-CHAT antibody
Host
Goat
Reactivity
Avian, Chicken, Guinea Pig, Human, Mouse, non-Human primates
Clonality
Polyclonal
Specificity
Specific for the ~ 70k choline acetyltransferase protein
Purity/Purification
Affinity Purified (Prepared from goat serum by affinity purification using a column to which the protein immunogen was coupled.)
Form/Format
100 ul in PBS + 5 mg/ml BSA + 0.2% NaN3.. Adequate amount of material to conduct 10-mini Western Blots.
Applicable Applications for anti-CHAT antibody
WB (Western Blot)
Antigen
Native choline acetyltransferase purified from human placenta
Immunogen Information
Native choline acetyltransferase purifed from human placenta
Immunogen Species
Human
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots with chicken, guinea pig, human, mouse, non-human primate and rat tissues.
Biological Significance
Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999).
Preparation and Storage
For long term storage -20 degree C is recommended. Stable for at least 1 year.
Application Data
Application Data
Related Product Information for anti-CHAT antibody
Affinity purified goat polyclonal antibody
References
• Karson CN, Casanova MF, Kleinman JE, Griffin WS (1993) Choline acetyltransferase in schizophrenia. Am. J.Psychiatry 150:454-459.
• Holt DJ, Herman MM, Hyde TM, Kleinman JE, Cinton CM, German DC, Hersh LB, Greybiel AM, Saper CB (1999) Evidence for a deficit in cholinergic interneurons in the striatum in schizophrenia. Neuroscience 94(1):21-31.
• Baskin DS, Browning JL, Pirozzolo FJ, Korporaal S, Baskin JA, Appel SH (1999) Brain choline acetyltransferase and mental function in Alzheimer disease. Arch Neurol. 56:1121-1123.
• Holt DJ, Herman MM, Hyde TM, Kleinman JE, Cinton CM, German DC, Hersh LB, Greybiel AM, Saper CB (1999) Evidence for a deficit in cholinergic interneurons in the striatum in schizophrenia. Neuroscience 94(1):21-31.
• Baskin DS, Browning JL, Pirozzolo FJ, Korporaal S, Baskin JA, Appel SH (1999) Brain choline acetyltransferase and mental function in Alzheimer disease. Arch Neurol. 56:1121-1123.
NCBI and Uniprot Product Information
NCBI GeneID
UniProt Accession #
Molecular Weight
70
NCBI Official Synonym Full Names
choline O-acetyltransferase
NCBI Official Symbol
CHAT
NCBI Official Synonym Symbols
CMS1A; CMS1A2; CHOACTASE
NCBI Protein Information
choline O-acetyltransferase; choline acetylase; acetyl CoA:choline O-acetyltransferase
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Product Notes
The CHAT (Catalog #AAA72808) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Choline Acetyltransferase reacts with Avian, Chicken, Guinea Pig, Human, Mouse, non-Human primates and may cross-react with other species as described in the data sheet. AAA Biotech's ChAT (Choline Acetyltransferase) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the CHAT for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ChAT (Choline Acetyltransferase), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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