Mouse Complement fragment 3a, C3a ELISA Kit | C3a elisa kit
Mouse Complement fragment 3a, C3a ELISA Kit
Reactivity
Mouse
Synonyms
Complement fragment 3a, C3a; N/A; Mouse Complement fragment 3a, C3a ELISA Kit; Mouse Complement fragment 3a; C3a ELISA Kit; Complement fragment 3a; C3a; C3a elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse C3a. No significant cross-reactivity or interference between mouse C3a and analogues was observed.
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.156 ng/ml-10 ng/ml
Sensitivity
< 0.039 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Related Product Information for C3a elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C3a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C3a present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C3a is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C3a bound in the initial step. The color development is stopped and the intensity of the color is measured.
