Canine Complement Fragment 3a ELISA Kit | C3a elisa kit

Canine Complement Fragment 3a ELISA Kit

Cat. Num. AAA17234

• Gene Names: C3; ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
• Reactivity: Canine
• Synonyms: Complement Fragment 3a; N/A; Canine Complement Fragment 3a ELISA Kit; C3a elisa kit

• Reactivity: Canine
• Specificity: This assay has high sensitivity and excellent specificity for detection of C3alpha. No significant cross-reactivity or interference between C3alpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C3alpha and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for C3a elisa kit

Principle of the Assay: C3alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-C3alpha antibody and an C3alpha-HRP conjugate. The assay sample and buffer are incubated together with C3alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C3alpha concentration since C3alpha from samples and C3alpha-HRP conjugate compete for the anti-C3alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by C3alpha from the sample, fewer sites are left to bind C3alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C3alpha concentration in each sample is interpolated from this standard curve.

Intended Uses: This C3alpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine C3alpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for C3a elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 115298678
• NCBI GeneID: 718
• NCBI Accession #: NP_000055.2
• NCBI GenBank Nucleotide #: NM_000064.2
• Molecular Weight: 187,148 Da
• NCBI Official Full Name: complement C3
• NCBI Official Synonym Full Names: complement component 3
• NCBI Official Symbol: C3
• NCBI Official Synonym Symbols: ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
• NCBI Protein Information: complement C3; prepro-C3; C3a anaphylatoxin; complement component C3; complement component C3a; complement component C3b; acylation-stimulating protein cleavage product; epididymis secretory sperm binding protein Li 62p; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
• UniProt Protein Name: Complement C3
• UniProt Gene Name: C3
• UniProt Synonym Gene Names: CPAMD1; ASP
• UniProt Entry Name: CO3_HUMAN