Mouse EGFR PicoKine ELISA Kit | Egfr elisa kit

Mouse EGFR PicoKine ELISA Kit

Cat. Num. AAA46127

• Gene Names: Egfr; Wa5; wa2; Erbb; Errp; wa-2; Errb1; AI552599; 9030024J15Rik
• Reactivity: Mouse
• Synonyms: EGFR PicoKine; N/A; Mouse EGFR PicoKine ELISA Kit; Epidermal growth factor receptor; EGF R; EGFR; erbb 1; Erbb1; erbb-1; HER1; PIG61; epidermal growth factor receptor; Egfr elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Egfr
• Sequence Length: 1210
• Samples: Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 156 pg/ml - 10,000 pg/ml
• Sensitivity: <15 pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Standard Curve (Sample)

Related Product Information for Egfr elisa kit

Background: The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGFalpha). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.

Principle of the Assay: The Picokine™ Mouse Egfr Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Egfr with a 96-well strip plate that is pre-coated with antibody specific for Egfr. The detection antibody is a biotinylated antibody specific for Egfr. The kit contains recombinant Mouse Egfr with immunogen: Expression system for standard: NS0; Immunogen sequence: L25-S647. The kit is analytically validated with ready-to-use reagents. To measure Mouse Egfr, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Egfr in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Egfr in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 46560582
• NCBI GeneID: 13649
• NCBI Accession #: NP_997538.1
• NCBI GenBank Nucleotide #: NM_207655.2
• UniProt Accession #: Q01279
• Molecular Weight: 134,853 Da
• NCBI Official Full Name: epidermal growth factor receptor isoform 1
• NCBI Official Synonym Full Names: epidermal growth factor receptor
• NCBI Official Symbol: Egfr
• NCBI Official Synonym Symbols: Wa5; wa2; Erbb; Errp; wa-2; Errb1; AI552599; 9030024J15Rik
• NCBI Protein Information: epidermal growth factor receptor
• UniProt Protein Name: Epidermal growth factor receptor
• UniProt Gene Name: Egfr
• UniProt Entry Name: EGFR_MOUSE