Mouse Fibroblast growth factor 15 (FGF15) ELISA Kit
Mouse Fibroblast growth factor 15, FGF15 ELISA Kit
Reactivity
Mouse
Synonyms
Fibroblast growth factor 15 (FGF15); N/A; Mouse Fibroblast growth factor 15, FGF15 ELISA Kit; Mouse Fibroblast growth factor 15 (FGF15) ELISA kit; FGF-15; Fibroblast growth factor 15 (FGF15) elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse FGF15. No significant cross-reactivity or interference between mouse FGF15 and analogues was observed.
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates, cell lysates
Detection Range
15.6 pg/ml-1000 pg/ml
Sensitivity
< 3.9 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Related Product Information for Fibroblast growth factor 15 (FGF15) elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FGF15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGF15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF15 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF15 bound in the initial step. The color development is stopped and the intensity of the color is measured.
