Mouse Growth Differentiation Factor 11 (GDF11) ELISA Kit | GDF11 elisa kit

Mouse Growth Differentiation Factor 11 (GDF11) High Sensitive ELISA Kit

Cat. Num. AAA188578

• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Growth Differentiation Factor 11 (GDF11); N/A; Mouse Growth Differentiation Factor 11 (GDF11) High Sensitive ELISA Kit; Growth Differentiation Factor 11; BMP11; Bone morphogenetic protein 11; GDF11 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Growth Differentiation Factor 11 (GDF11).; No significant cross-reactivity or interference between High Sensitive Growth Differentiation Factor 11 (GDF11) and analogues was observed.
• Sequence Length: 405
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 6.25-400pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 2.21pg/mL

• Applicable Applications for GDF11 elisa kit: ELISA
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Growth Differentiation Factor 11 (GDF11) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Growth Differentiation Factor 11 (GDF11) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Growth Differentiation Factor 11 (GDF11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Growth Differentiation Factor 11 (GDF11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Growth Differentiation Factor 11 (GDF11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Growth Differentiation Factor 11 (GDF11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

NCBI and Uniprot Product Information

• NCBI GI #: 112807180
• NCBI Accession #: NP_034402.1
• NCBI GenBank Nucleotide #: NP_034402.1
• UniProt Accession #: Q9Z1W4
• NCBI Official Full Name: growth/differentiation factor 11 preproprotein
• UniProt Protein Name: Growth/differentiation factor 11
• UniProt Gene Name: Gdf11
• UniProt Synonym Gene Names: Bmp11; GDF-11; BMP-11