Mouse Inter-alpha-trypsin inhibitor heavy chain H2 (ITIH2) ELISA Kit | ITIH2 elisa kit

Mouse Inter-alpha-trypsin inhibitor heavy chain H2 (ITIH2) ELISA Kit

Cat. Num. AAA246390

• Gene Names: ITIH2; H2P; SHAP
• Reactivity: Mouse
• Synonyms: Inter-alpha-trypsin inhibitor heavy chain H2 (ITIH2); N/A; Mouse Inter-alpha-trypsin inhibitor heavy chain H2 (ITIH2) ELISA Kit; ITIH2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of ITIH2. No significant cross-reactivity or interference between ITIH2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ITIH2 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ug/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for ITIH2 elisa kit

Principle of the Assay: ITIH2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ITIH2 antibody and an ITIH2-HRP conjugate. The assay sample and buffer are incubated together with ITIH2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ITIH2 concentration since ITIH2 from samples and ITIH2-HRP conjugate compete for the anti-ITIH2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ITIH2 from the sample, fewer sites are left to bind ITIH2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ITIH2 concentration in each sample is interpolated from this standard curve.

Intended Uses: This ITIH2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ITIH2. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for ITIH2 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 70778918
• NCBI GeneID: 3698
• NCBI Accession #: NP_002207.2
• NCBI GenBank Nucleotide #: NM_002216.2
• Molecular Weight: 106,463 Da
• NCBI Official Full Name: inter-alpha-trypsin inhibitor heavy chain H2
• NCBI Official Synonym Full Names: inter-alpha-trypsin inhibitor heavy chain 2
• NCBI Official Symbol: ITIH2
• NCBI Official Synonym Symbols: H2P; SHAP
• NCBI Protein Information: inter-alpha-trypsin inhibitor heavy chain H2; ITI-HC2; ITI heavy chain H2; inter-alpha (globulin) inhibitor H2; inter-alpha-inhibitor heavy chain 2; serum-derived hyaluronan-associated protein; inter-alpha (globulin) inhibitor, H2 polypeptide; inter-alpha-trypsin inhibitor complex component II
• UniProt Protein Name: Inter-alpha-trypsin inhibitor heavy chain H2
• UniProt Gene Name: ITIH2
• UniProt Synonym Gene Names: IGHEP2; ITI heavy chain H2; ITI-HC2; Inter-alpha-inhibitor heavy chain 2; SHAP
• UniProt Entry Name: ITIH2_HUMAN