IF (Immunofluorescence)
(AAA329029 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degrees C. Samples were then incubated with primary Ab(AAA329029 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37 degrees C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Rabbit ITIH2 Polyclonal Antibody | anti-ITIH2 antibody
ITIH2 Antibody
Gene Names
ITIH2; H2P; SHAP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Synonyms
ITIH2, Antibody; ITIH2 Antibody; AI747202; H2P; IGHEP2; Inter alpha (globulin) inhibitor H2; Inter alpha globulin inhibitor H2 polypeptide; Inter alpha trypsin inhibitor complex component II; Inter alpha trypsin inhibitor heavy chain H2; Inter-alpha-inhibitor heavy chain 2; Inter-alpha-trypsin inhibitor complex component II; Inter-alpha-trypsin inhibitor heavy chain H2; Intin2; ITI HC2; ITI heavy chain H2; ITI-HC2; Itih 2; Itih2; ITIH2_HUMAN; OTTHUMP00000019058; OTTHUMP00000019059; OTTMUSP00000012019; RP23 119N4.3; Serum derived hyaluronan associated protein; Serum-derived hyaluronan-associated protein; SHAP; anti-ITIH2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Form/Format
Supplied in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
~1mg/ml (varies by lot)
Sequence Length
946
Applicable Applications for anti-ITIH2 antibody
WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Immunogen
A synthesized peptide derived from human ITIH2, corresponding to a region within C-terminal amino acids.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA329029 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degrees C. Samples were then incubated with primary Ab(AAA329029 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37 degrees C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA329029 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degrees C. Samples were then incubated with primary Ab(AAA329029 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37 degrees C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IHC (Immunohiostchemistry)
(AAA329029 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degrees C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA329029 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degrees C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from B16F10 cells, using ITIH2 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from B16F10 cells, using ITIH2 Antibody. The lane on the left was treated with blocking peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
106kDa
NCBI Official Full Name
inter-alpha-trypsin inhibitor heavy chain H2
NCBI Official Synonym Full Names
inter-alpha-trypsin inhibitor heavy chain 2
NCBI Official Symbol
ITIH2
NCBI Official Synonym Symbols
H2P; SHAP
NCBI Protein Information
inter-alpha-trypsin inhibitor heavy chain H2
UniProt Protein Name
Inter-alpha-trypsin inhibitor heavy chain H2
UniProt Gene Name
ITIH2
UniProt Synonym Gene Names
IGHEP2; ITI heavy chain H2; ITI-HC2; Inter-alpha-inhibitor heavy chain 2; SHAP
UniProt Entry Name
ITIH2_HUMAN
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Product Notes
The ITIH2 itih2 (Catalog #AAA329029) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ITIH2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ITIH2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the ITIH2 itih2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ITIH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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