Mouse Keratin, type I cytoskeletal 18 fragment (K18-Asp396) ELISA Kit | K18-Asp396 elisa kit
Mouse Keratin, type I cytoskeletal 18 fragment (K18-Asp396) ELISA Kit
Reactivity
Mouse
Synonyms
Keratin, type I cytoskeletal 18 fragment (K18-Asp396); N/A; Mouse Keratin, type I cytoskeletal 18 fragment (K18-Asp396) ELISA Kit; K18-Asp396 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse K18-Asp396. No significant cross-reactivity or interference between mouse K18-Asp396 and analogues was observed.
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates
Detection Range
15.6 uIU/ml-1000 uIU/ml
Sensitivity
< 3.9 uIU/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for K18-Asp396 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for K18-Asp396 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any K18-Asp396 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for K18-Asp396 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of K18-Asp396 bound in the initial step. The color development is stopped and the intensity of the color is measured.