Mouse Matrix Metalloproteinase 13 (MMP-13) ELISA Kit | MMP-13 elisa kit

Mouse Matrix Metalloproteinase 13 (MMP-13) ELISA Kit

Cat. Num. AAA332410

• Gene Names: Mmp13; Clg; Mmp1; MMP-13
• Reactivity: Mouse
• Synonyms: Matrix Metalloproteinase 13 (MMP-13); N/A; Mouse Matrix Metalloproteinase 13 (MMP-13) ELISA Kit; MMP13; CLG3; MANDP1; collagenase 3; matrix metalloproteinase 13; matrix metalloproteinase 13 (collagenase 3); MMP-13 elisa kit

• Reactivity: Mouse
• Specificity: Mouse Matrix metalloproteinase 13 (MMP-13) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MMP13. No significant cross-reactivity or interference between Mouse MMP13 and analogues was observed.
• Sequence Length: 472
• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids

• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Standard Curve (Sample)

(Fig.1. Mouse Matrix metalloproteinase 13 (MMP-13) Standard Curve.)

Related Product Information for MMP-13 elisa kit

Description: This Mouse Matrix metalloproteinase 13 (MMP-13) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP13 in samples. An antibody specific for MMP13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP13 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Mmp13 encodes a member of the matrix metalloproteinase family that plays a role in wound healing, skeletal development and bone remodeling. The encoded protein is activated by the removal of an N-terminal activation peptide to generate a zinc-dependent endopeptidase enzyme that can cleave various native collagens, including types I - IV, X and XIV. Mice lacking the encoded protein display profound defects in growth plate cartilage as well as a delay in the endochondral bone development. Lack of the encoded protein also impairs the wound healing process due to reduced keratinocyte migration and vascular density at the wound site. Mmp13 is located in a cluster of other matrix metalloproteinase genes on chromosome 9.

NCBI and Uniprot Product Information

• NCBI GI #: 6678896
• NCBI GeneID: 17386
• NCBI Accession #: NP_032633.1
• NCBI GenBank Nucleotide #: NP_032633.1
• UniProt Accession #: P33435
• NCBI Official Full Name: collagenase 3 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 13
• NCBI Official Symbol: Mmp13
• NCBI Official Synonym Symbols: Clg; Mmp1; MMP-13
• NCBI Protein Information: collagenase 3
• UniProt Protein Name: Collagenase 3
• UniProt Gene Name: Mmp13
• UniProt Synonym Gene Names: MMP-13