Mouse MT ELISA Kit | MT elisa kit
Mouse MT (Melatonin) ELISA Kit
Reactivity
Mouse
Synonyms
MT; N/A; Mouse MT (Melatonin) ELISA Kit; MT elisa kit
Reactivity
Mouse
Specificity
This kit recognizes Mouse MT in samples. No significant cross-reactivity or interference between Mouse MT and analogues was observed.
Sequence Length
60
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Competitive
Detection Range
7.81-500pg/mL
Sensitivity
4.69pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse MT were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse MT were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Standard Curve (Sample)
Related Product Information for MT elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse MT concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse MT. During the reaction, Mouse MT in the sample or standard competes with a fixed amount of Mouse MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse MT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Mouse MT in the samples is then determined by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse MT. During the reaction, Mouse MT in the sample or standard competes with a fixed amount of Mouse MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse MT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Mouse MT in the samples is then determined by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
6,007 Da
NCBI Official Full Name
Metallothionein
NCBI Official Symbol
LOC100049397
NCBI Protein Information
metallothionein; MT
UniProt Protein Name
Metallothionein
UniProt Gene Name
mt
UniProt Synonym Gene Names
MT
UniProt Entry Name
MT_ORYLA
