Mouse NADH-ubiquinone oxidoreductase chain 6 (MT-ND6) ELISA Kit | MT-ND6 elisa kit
Mouse NADH-ubiquinone oxidoreductase chain 6 (MT-ND6) ELISA Kit
Reactivity
Mouse
Synonyms
NADH-ubiquinone oxidoreductase chain 6 (MT-ND6); N/A; Mouse NADH-ubiquinone oxidoreductase chain 6 (MT-ND6) ELISA Kit; MT-ND6 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of MTND6. No significant cross-reactivity or interference between MTND6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MTND6 and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for MT-ND6 elisa kit
Principle of the Assay: MTND6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MTND6 antibody and an MTND6-HRP conjugate. The assay sample and buffer are incubated together with MTND6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MTND6 concentration since MTND6 from samples and MTND6-HRP conjugate compete for the anti-MTND6 antibody binding site. Since the number of sites is limited, as more sites are occupied by MTND6 from the sample, fewer sites are left to bind MTND6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MTND6 concentration in each sample is interpolated from this standard curve.
Intended Uses: This MTND6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MTND6. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This MTND6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MTND6. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
22,276 Da
NCBI Official Full Name
NADH-ubiquinone oxidoreductase chain 6
UniProt Protein Name
NADH-ubiquinone oxidoreductase chain 6
UniProt Entry Name
B6BWX8_9PROT
