Standard Curve (Sample)
(Mouse NGFR PicoKine ELISA Kit standard curve)
Mouse NGFR/TNFRSF16 ELISA Kit | NGFR elisa kit
Mouse NGFR/TNFRSF16 ELISA Kit PicoKine
Gene Names
Ngfr; p75; LNGFR; p75NTR; p75NGFR; Tnfrsf16
Reactivity
Mouse
Applications
ELISA
Synonyms
NGFR/TNFRSF16; N/A; Mouse NGFR/TNFRSF16 ELISA Kit PicoKine; Nt5e; Nt5; Nte; 5'-nucleotidase; 5'-NT; EC 3.1.3.5; Ecto-5'-nucleotidase; CD antigen CD73; nerve growth factor receptor (TNFR superfamily, member 16); NGFR elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Ngfr
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
31.2 pg/ml - 2,000 pg/ml
Sensitivity
<10 pg/ml
Applicable Applications for NGFR elisa kit
ELISA
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Standard Curve (Sample)
(Mouse NGFR PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Mouse NGFR PicoKine ELISA Kit standard curve)
Related Product Information for NGFR elisa kit
Background: Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5. The nerve growth factor receptor gene is at human chromosome region 17q12-17q22, distal to the chromosome 17 breakpoint in acute leukemias. The neurotrophin receptor p75(NTR), a tumor necrosis factor receptor superfamily member expressed in hepatic stellate cells after fibrotic and cirrhotic liver injury in humans, is a regulator of liver repair. In mice, depletion of p75(NTR) exacerbated liver pathology and inhibited hepatocyte proliferation in vivo. In addition, it is showed that neurotrophins activate p75(NTR) to induce apoptosis through the induction of the sphingomyelin (SM) cycle and increased production of ceramide. Overexpression of p75(NTR) is also found to activate the SM pathway.
Principle of the Assay: The Picokine™ Mouse Ngfr Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Ngfr with a 96-well strip plate that is pre-coated with antibody specific for Ngfr. The detection antibody is a biotinylated antibody specific for Ngfr. The capture antibody is a monoclonal antibody from rat and the detection antibody is a biotinylated polyclonal antibody from goat. The kit includes Mouse Ngfr protein as standards. To measure Mouse Ngfr, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Ngfr in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Ngfr in the sample.
Principle of the Assay: The Picokine™ Mouse Ngfr Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Ngfr with a 96-well strip plate that is pre-coated with antibody specific for Ngfr. The detection antibody is a biotinylated antibody specific for Ngfr. The capture antibody is a monoclonal antibody from rat and the detection antibody is a biotinylated polyclonal antibody from goat. The kit includes Mouse Ngfr protein as standards. To measure Mouse Ngfr, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Ngfr in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Ngfr in the sample.
Product Categories/Family for NGFR elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
50kDa
NCBI Official Full Name
Tumor necrosis factor receptor superfamily member 16
NCBI Official Synonym Full Names
nerve growth factor receptor (TNFR superfamily, member 16)
NCBI Official Symbol
Ngfr
NCBI Official Synonym Symbols
p75; LNGFR; p75NTR; p75NGFR; Tnfrsf16
NCBI Protein Information
tumor necrosis factor receptor superfamily member 16
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 16
UniProt Gene Name
Ngfr
UniProt Synonym Gene Names
Tnfrsf16; NGF receptor
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Product Notes
The Mouse NGFR ngfr (Catalog #AAA126676) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA126676 ELISA Kit recognizes Mouse NGFR. AAA Biotech's NGFR/TNFRSF16 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the NGFR ngfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NGFR/TNFRSF16, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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