Standard Curve (Sample)
Mouse ON (Osteonectin) ELISA Kit | ON elisa kit
Mouse ON (Osteonectin) ELISA Kit
Reactivity
Mouse
Synonyms
ON (Osteonectin); N/A; Mouse ON (Osteonectin) ELISA Kit; SPARC; BM-40; Secreted Protein,Acidic,Cysteine-Rich; Basement-membrane protein 40; Secreted protein acidic and rich in cysteine; ON elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse ON. No significant cross-reactivity or interference between Mouse ONand analogues was observed.
Assay Type
Sandwich
Detection Range
0.16-10 ng/mL
Sensitivity
0.059 ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay)? CV% < 8%. Three samples of known concentration were tested twenty times on 1 plate to assess intra-assay precision.
Inter-assay Precision
Inter-assay Precision (precision between assays)? CV% < 10%. Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Preparation and Storage
Store at 2-8 degree C. Stable for 6 months from date of receipt.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ON elisa kit
Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ON. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ON. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ON, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ON in the samples is then determined by comparing the OD of the samples to the standard curve.
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Product Notes
The Mouse ON (Catalog #AAA27991) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA27991 ELISA Kit recognizes Mouse ON. It is sometimes possible for the material contained within the vial of "ON (Osteonectin), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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