Reactivity
Mouse
Assay Type
Competitive
Samples
Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids
Detection Range
3-900ng/L
Sensitivity
1.27ng/L
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV< 10%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV< 12%
Related Product Information for RA elisa kit
Principle of the Assay: This kit is a Enzyme-Linked Immunosorbent Assay (ELISA). Add samples to the pre-coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin-HRP is then added and then incubate. Unbound avidin-HRP is washed away during a washing step. TMB Substrate is then added and color develops. The reaction is stopped by addition of acidic stop solution and color changes into yellow that can be measured at 450 nm. The intensity of the color developed is inversely proportional to the concentration of RA in the sample. The concentration of RA in the sample is then determined by comparing the O.D. of the samples to the standard curve.
Intended Uses: This competitive ELISA kit is for the quantification of Retinoic Acid(also known as RA) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Intended Uses: This competitive ELISA kit is for the quantification of Retinoic Acid(also known as RA) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.