Standard Curve (Sample)
(Fig.1. Mouse Serine/threonine-protein kinase 11 (STK11) Standard Curve.)
Mouse Serine/Threonine-Protein Kinase 11 (STK11) ELISA Kit | STK11 elisa kit
Mouse Serine/Threonine-Protein Kinase 11 (STK11) ELISA Kit
Gene Names
Stk11; Lkb1; Par-4; R75140; AA408040
Reactivity
Mouse
Synonyms
Serine/Threonine-Protein Kinase 11 (STK11); N/A; Mouse Serine/Threonine-Protein Kinase 11 (STK11) ELISA Kit; STK11; LKB1; PJS; polarization-related protein LKB1; serine/threonine protein kinase 11; STK11 elisa kit
Reactivity
Mouse
Specificity
Mouse Serine/threonine-protein kinase 11 (STK11) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse STK11. No significant cross-reactivity or interference between Mouse STK11 and analogues was observed.
Sequence Length
412
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Mouse Serine/threonine-protein kinase 11 (STK11) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Mouse Serine/threonine-protein kinase 11 (STK11) Standard Curve.)
Related Product Information for STK11 elisa kit
Description: This Mouse Serine/threonine-protein kinase 11 (STK11) ELISA Kit employs a two-site sandwich ELISA to quantitate STK11 in samples. An antibody specific for STK11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any STK11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STK11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STK11 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: LKB1 is a primary upstream kinase of adenine monophosphate-activated protein kinase (AMPK), a necessary element in cell metabolism that is required for maintaining energy homeostasis. It is now clear that LKB1 exerts its growth suppressing effects by activating a group of other ~14 kinases, comprising AMPK and AMPK-related kinases. Activation of AMPK by LKB1 suppresses growth and proliferation when energy and nutrient levels are scarce. Activation of AMPK-related kinases by LKB1 plays vital roles maintaining cell polarity thereby inhibiting inappropriate expansion of tumour cells. A picture from current research is emerging that loss of LKB1 leads to disorganization of cell polarity and facilitates tumour growth under energetically unfavorable conditions.
Background: LKB1 is a primary upstream kinase of adenine monophosphate-activated protein kinase (AMPK), a necessary element in cell metabolism that is required for maintaining energy homeostasis. It is now clear that LKB1 exerts its growth suppressing effects by activating a group of other ~14 kinases, comprising AMPK and AMPK-related kinases. Activation of AMPK by LKB1 suppresses growth and proliferation when energy and nutrient levels are scarce. Activation of AMPK-related kinases by LKB1 plays vital roles maintaining cell polarity thereby inhibiting inappropriate expansion of tumour cells. A picture from current research is emerging that loss of LKB1 leads to disorganization of cell polarity and facilitates tumour growth under energetically unfavorable conditions.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
serine/threonine-protein kinase STK11 isoform S
NCBI Official Synonym Full Names
serine/threonine kinase 11
NCBI Official Symbol
Stk11
NCBI Official Synonym Symbols
Lkb1; Par-4; R75140; AA408040
NCBI Protein Information
serine/threonine-protein kinase STK11
UniProt Protein Name
Serine/threonine-protein kinase STK11
UniProt Gene Name
Stk11
UniProt Synonym Gene Names
LKB1; mLKB1
Similar Products
Product Notes
The Mouse STK11 stk11 (Catalog #AAA332280) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332280 ELISA Kit recognizes Mouse STK11. It is sometimes possible for the material contained within the vial of "Serine/Threonine-Protein Kinase 11 (STK11), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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