Standard Curve (Sample)
Mouse Spondin-2 (SPON2) ELISA Kit | SPON2 elisa kit
Mouse Spondin-2 (SPON2) ELISA Kit
Gene Names
Spon2; Mindin; AI504350; Mspondin; M-spondin; 2310045I24Rik
Reactivity
Mouse
Synonyms
Spondin-2 (SPON2); N/A; Mouse Spondin-2 (SPON2) ELISA Kit; SPON2; DIL-1; DIL1; DKFZp686G21139; FLJ16313; FLJ34460; M-spondin; Mindin; SPON2 elisa kit
Reactivity
Mouse
Specificity
Mouse Spondin-2 (SPON2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPON2. No significant cross-reactivity or interference between Mouse SPON2 and analogues was observed.
Sequence Length
330
Assay Type
Sandwich
Samples
Mouse serum, cell culture supernates and other biological fluids
Detection Range
20 ng/L - 320 ng/L
Sensitivity
< 1ng/L
Intra-assay Precision
Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SPON2 elisa kit
Background: The full-length SPON2 cDNA encodes a 331-amino acid protein with a domain organization similar to those of zebrafish mindin-1/mindin-2 and F-spondin (SPON1): a hydrophobic signal sequence in the N terminus, an FS1 domain, an FS2 domain, and a thrombospondin type I repeat.The mouse mindin protein, which is 85% identical to the human protein, is a conserved member of a family of secreted extracellular matrix proteins. RNA blot analysis detected expression in a variety of tissues, with abundant expression in lung and lymphoid tissues.
Principle of the Assay: Mouse Spondin-2 (SPON2) ELISA Kit employs a two-site sandwich ELISA to quantitate SPON2 in samples. An antibody specific for SPON2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPON2 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Mouse SPON2 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of SPON2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: Mouse Spondin-2 (SPON2) ELISA Kit employs a two-site sandwich ELISA to quantitate SPON2 in samples. An antibody specific for SPON2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPON2 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Mouse SPON2 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of SPON2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
spondin-2
NCBI Official Synonym Full Names
spondin 2, extracellular matrix protein
NCBI Official Symbol
Spon2
NCBI Official Synonym Symbols
Mindin; AI504350; Mspondin; M-spondin; 2310045I24Rik
NCBI Protein Information
spondin-2
UniProt Protein Name
Spondin-2
UniProt Gene Name
Spon2
UniProt Entry Name
SPON2_MOUSE
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Product Notes
The Mouse SPON2 spon2 (Catalog #AAA332251) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332251 ELISA Kit recognizes Mouse SPON2. It is sometimes possible for the material contained within the vial of "Spondin-2 (SPON2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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