Human spondin 2, extracellular matrix protein ELISA Kit | SPON2 elisa kit
Human Spondin-2 ELISA Kit
Gene Names
SPON2; DIL1; DIL-1; MINDIN; M-SPONDIN
Reactivity
Human
Synonyms
spondin 2, extracellular matrix protein; N/A; Human Spondin-2 ELISA Kit; DIL-1; DIL1; DKFZp686G21139; FLJ16313; FLJ34460; M-spondin; Mindin; ; spondin 2; extracellular matrix protein; SPON2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human spondin-2. No significant cross-reactivity or interference between human spondin-2 and analogues was observed.
Sequence Length
331
Samples
Serum, plasma, cerebrospinal fluid (CSF), cell culture supernates
Assay Type
Sandwich
Detection Range
3.12 ng/ml -200 ng/ml
Sensitivity
0.78 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for SPON2 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for spondin-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any spondin-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for spondin-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of spondin-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,846 Da
NCBI Official Full Name
spondin-2
NCBI Official Synonym Full Names
spondin 2, extracellular matrix protein
NCBI Official Symbol
SPON2
NCBI Official Synonym Symbols
DIL1; DIL-1; MINDIN; M-SPONDIN
NCBI Protein Information
spondin-2; differentially expressed in cancerous and non-cancerous lung cells 1
UniProt Protein Name
Spondin-2
UniProt Gene Name
SPON2
UniProt Synonym Gene Names
DIL1; UNQ435/PRO866; DIL-1
UniProt Entry Name
SPON2_HUMAN