Application Data
(The OmniKine Murine TNF-alpha ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Murine TNF-alpha proteins within the range of 16-2000 pg/ml.)
Mouse TNF-alpha ELISA Kit | TNF-alpha elisa kit
TNF-alpha (Murine) OmniKine ELISA Kit
Gene Names
Tnf; DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha
Reactivity
Mouse
Synonyms
TNF-alpha; N/A; TNF-alpha (Murine) OmniKine ELISA Kit; Tumor necrosis factor; Cachectin; Tumor necrosis factor ligand superfamily member 2; TNFSF2; TNF-alpha elisa kit
Reactivity
Mouse
Specificity
The OmniKine™ Murine TNF-alpha ELISA is capable of recognizing both recombinant and naturally produced Murine TNF-alpha proteins. The antigens listed below were tested at 50 ng/ml and exhibited 100% cross reactivity.
Form/Format
12 x 8-Well Microstrips
Sequence Length
219
Assay Type
Quantitative Sandwich
Sensitivity
32-2000 pg/ml
Detection Method
Colorimetric 450 nm
Subtype
None
Preparation and Storage
Store entire kit at 4 degree C until use.
Application Data
(The OmniKine Murine TNF-alpha ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Murine TNF-alpha proteins within the range of 16-2000 pg/ml.)
Application Data
(The OmniKine Murine TNF-alpha ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Murine TNF-alpha proteins within the range of 16-2000 pg/ml.)
Related Product Information for TNF-alpha elisa kit
Background: Murine TNF-alpha or Tumor Necrosis Factor alpha, also known as Cachectin, is a 235 amino acid cytokine protein expressed from the Tnf gene located on chromosome 17. The 235 residue chain exists primarily as the membrane form of TNF-alpha whereas the 156 residue form, created by proteolytic processing from the N-terminal end, exists primarily as the soluble form. The membrane form contains a 35 residue cytoplasmic topological domain, a 21 residue helical membrane domain, and a 179 residue extracellular topological domain. TNF-alpha is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. The cytokine is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia. Under certain conditions, it can stimulate cell proliferation and induce cell differentiation.
Principle of the Assay: The OmniKine™ Murine TNF-alpha ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Murine TNF-alpha concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Murine TNF-alpha while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Principle of the Assay: The OmniKine™ Murine TNF-alpha ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Murine TNF-alpha concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Murine TNF-alpha while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,896 Da
NCBI Official Full Name
tumor necrosis factor isoform 2
NCBI Official Synonym Full Names
tumor necrosis factor
NCBI Official Symbol
Tnf
NCBI Official Synonym Symbols
DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha
NCBI Protein Information
tumor necrosis factor
UniProt Protein Name
Tumor necrosis factor
UniProt Gene Name
Tnf
UniProt Synonym Gene Names
Tnfa; Tnfsf2; TNF-a; NTF; ICD1; ICD2
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Product Notes
The Mouse TNF-alpha tnf (Catalog #AAA315690) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA315690 ELISA Kit recognizes Mouse TNF-alpha. It is sometimes possible for the material contained within the vial of "TNF-alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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