Mouse TNFRSF13B/TACI ELISA Kit | TNFRSF13B elisa kit

Mouse TNFRSF13B/TACI PicoKine ELISA Kit

Cat. Num. AAA45749

• Gene Names: Tnfrsf13b; Taci; 1200009E08Rik
• Reactivity: Mouse
• Synonyms: TNFRSF13B/TACI; N/A; Mouse TNFRSF13B/TACI PicoKine ELISA Kit; Tumor necrosis factor receptor superfamily member 13B; tumor necrosis factor receptor superfamily, member 13B; CD 267; CD267; CD267 antigen; CVID; CVID2; FLJ39942; MGC133214; MGC39952; OTTHUMP00000065442; TNFRSF 13B; TNFRSF 14B; TNFRSF13B; TNFRSF13B protein; TNFRSF14B; TR13B_HUMAN; Transmembrane activator and CAML interactor; Tumor necrosis factor receptor 13B; TACI; TNFRSF13B elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse TNFRSF13B
• Sequence Length: 249
• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 46.9pg/ml-3000pg/ml
• Sensitivity: < 10pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Mouse TNFRSF13B/TACI PicoKine ELISA Kit standard curve)

Related Product Information for TNFRSF13B elisa kit

Principle of the assay: mouse TNFRSF13B ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF13B has been precoated onto 96-well plates. Standards(NSO, A2-C128) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF13B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF13B amount of sample captured in plate.
Background: Tumor necrosis factor receptor superfamily member 13B, also known as TNFRSF13B or more commonly as TACI, is a transmembrane receptor protein found predominantly on the surface of B cells, which are an important part of the immune system. TACI controls T cell-independent B cell antibody responses, isotype switching, and B cell homeostasis. TACI is a lymphocyte-specific member of the tumor necrosis factor (TNF) receptor superfamily. It was originally discovered because of its ability to interact with calcium-modulator and cyclophilin ligand (CAML). TACI was later found to play a crucial role in humoral immunity by interacting with two members of the TNF family: BAFF and APRIL. These proteins signal through TACI inducing activation of several transcription factors including NFAT, AP-1, and NF-kappa-B which then modulate cellular activities. Defects in the function of TACI can lead to immune system diseases.

NCBI and Uniprot Product Information

• NCBI GI #: 21264099
• NCBI GeneID: 57916
• UniProt Accession #: Q9ET35; Q9DBZ3
• Molecular Weight: 26,947 Da
• NCBI Official Full Name: Tumor necrosis factor receptor superfamily member 13B
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 13b
• NCBI Official Symbol: Tnfrsf13b
• NCBI Official Synonym Symbols: Taci; 1200009E08Rik
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 13B; transmembrane activator and CAML interactor; transmembrane activator and calcium-modulating and cyclophilin ligand interactor
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 13B
• UniProt Gene Name: Tnfrsf13b
• UniProt Synonym Gene Names: Taci
• UniProt Entry Name: TR13B_MOUSE